Human tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

中文名： 人基质金属蛋白酶抑制因子2(TIMP-2)ELISA Kit

货号: IPD-E04733h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIMP2含量。TIMP2即金属蛋白酶组织抑制因子 2，它在调控细胞外基质代谢、抑制基质金属蛋白酶活性方面有重要作用。其与肿瘤的发生、发展、侵袭和转移密切相关。研究多聚焦于它在肿瘤微环境中的作用机制，以及作为潜在治疗靶点的应用。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。支持科研人员开展组织微环境分析、疾病模型机制研究或药物干预效果评估，适用于心血管疾病、肿瘤生物学、肝肺纤维化等领域的体外实验需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CSC 21K ELISA Kit; CSC-21K ELISA Kit; CSC21K ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Metalloproteinase inhibitor 2 precursor ELISA Kit; TIMP 2 ELISA Kit; TIMP metallopeptidase inhibitor 2 ELISA Kit; TIMP-2 ELISA Kit; TIMP2 ELISA Kit; TIMP2_HUMAN ELISA Kit; Tissue Inhibitor of Metalloproteinase 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit
缩写：
TIMP2
Uniprot No.：
P16035
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)627.044626.079625.114625.693622.218625.693SD0.0310.0350.0290.0410.0470.044CV(%)4.2474.8013.9845.6276.4836.039

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:20

Average %

Range %

90-102

1:40

Average %

Range %

89-99

1:80

Average %

Range %

82-99

1:160

Average %

Range %

85-97

回收率：

The recovery of human TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.812	2.755	2.784	2.627
2500	2.212	2.125	2.169	2.012
1250	1.384	1.307	1.346	1.189
625	0.733	0.789	0.761	0.604
312	0.384	0.365	0.375	0.218
156	0.298	0.277	0.288	0.131
78	0.236	0.226	0.231	0.074
0	0.158	0.156	0.157