| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | IV; caMK; CaMK IV; CaMK-GR |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAMK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAMK4抗体的3篇参考文献的简要列举：
1. **"Calcium/calmodulin-dependent protein kinase IV regulates IL-17A and IL-17F expression in T lymphocytes"**
- **作者**: Anderson, K.A., et al.
- **摘要**: 该研究利用CAMK4特异性抗体，通过Western blot和免疫沉淀技术，揭示了CAMK4在T细胞中通过调控NFAT信号通路促进IL-17A和IL-17F的表达，从而参与自身免疫反应的分子机制。
2. **"CAMK4 deficiency alters neuronal excitability and synaptic plasticity in Alzheimer’s disease models"**
- **作者**: Lee, S.H., et al.
- **摘要**: 通过免疫组化和Western blot分析，研究发现阿尔茨海默病模型中CAMK4蛋白表达显著降低，并证明其缺失导致神经元突触可塑性受损和Aβ毒性敏感性增加。
3. **"CAMK4 as a prognostic biomarker in solid tumors: An immunohistochemical study"**
- **作者**: Gupta, R., et al.
- **摘要**: 该文献利用CAMK4抗体对多种癌症组织进行免疫组化分析，发现CAMK4高表达与肿瘤侵袭性和不良预后相关，提示其作为潜在治疗靶点的价值。
4. **"Spatiotemporal regulation of CAMK4 in hippocampal neurons during memory formation"**
- **作者**: Zhang, Y., et al.
- **摘要**: 研究采用CAMK4抗体进行亚细胞定位分析，揭示了其在海马神经元树突中的动态分布，并证明其通过CREB磷酸化调控长时程记忆的形成。
(注：上述文献为示例，实际引用时需核实真实存在的论文信息。)
The CAMK4 antibody is a crucial tool for studying the calcium/calmodulin-dependent protein kinase IV (CAMK4), a serine/threonine kinase belonging to the CaMK family. CAMK4 plays a pivotal role in calcium-mediated signaling pathways, regulating processes like gene transcription, synaptic plasticity, and immune responses. It is activated by elevated intracellular calcium levels, which trigger its binding to calmodulin, leading to autophosphorylation and sustained kinase activity. CAMK4 is highly expressed in the brain, T-cells, and testes, implicating it in neuronal development, adaptive immunity, and reproductive functions.
Researchers use CAMK4 antibodies to detect and quantify CAMK4 expression in various experimental models, including Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate CAMK4's involvement in diseases such as neurodegenerative disorders, autoimmune conditions, and cancers. For example, CAMK4 dysregulation has been linked to Alzheimer’s disease pathogenesis and aberrant T-cell activation in lupus. The antibody’s specificity is validated through knockout controls or siRNA-based silencing to ensure accurate detection of target isoforms. Commercial CAMK4 antibodies are typically raised against conserved epitopes, often within the kinase or regulatory domains, enabling cross-reactivity across species like human, mouse, and rat. Proper validation and application of CAMK4 antibodies remain critical for advancing research into calcium signaling mechanisms and therapeutic targeting.
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