| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CENP-P; RP11-19J3.3 |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CENPP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于CENPP(Centromere Protein P)抗体的模拟参考文献示例(实际文献需根据具体数据库检索)：
1. **文献名称**: "CENPP is a conserved centromere protein essential for chromosome segregation"
**作者**: Brown, K.E. et al.
**摘要**: 本研究通过CRISPR筛选发现CENPP在人类细胞中参与着丝粒复合体组装，利用特异性抗体证实其与CENP-A/CENP-C的相互作用，揭示其在染色体分离中的关键作用。
2. **文献名称**: "Antibody-based profiling of CENPP in colorectal cancer progression"
**作者**: Zhang, L. et al.
**摘要**: 开发了高特异性CENPP单克隆抗体，发现其在结直肠癌组织中过表达，通过免疫组化分析表明CENPP表达水平与患者预后不良相关，提示其作为癌症生物标志物的潜力。
3. **文献名称**: "Structural insights into the CENPP-CENPQ complex by cryo-EM"
**作者**: Tanaka, Y. et al.
**摘要**: 使用CENPP抗体纯化内源性着丝粒复合体，结合冷冻电镜解析CENPP与CENPQ的三维结构，阐明二者在维持着丝粒稳定性中的协同机制。
4. **文献名称**: "CENPP dysfunction leads to mitotic errors in primary microcephaly models"
**作者**: Gupta, R. et al.
**摘要**: 在小鼠模型中通过CENPP抗体检测发现，CENPP缺失导致有丝分裂纺锤体异常和神经元增殖缺陷，提示其突变可能与原发性小头症相关。
---
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索确认。建议使用关键词“CENPP antibody”或“CENP-P function”结合过滤器(如“Review”“Molecular Biology”)查找。
CENPP (Centromere Protein P) is a key component of the centromere, a specialized chromosomal region essential for proper chromosome segregation during cell division. As part of the constitutive centromere-associated network (CCAN), CENPP interacts with other centromeric proteins, such as CENPA, CENPC, and CENPH, to maintain the structural and functional integrity of the centromere. It plays a critical role in kinetochore assembly, ensuring accurate microtubule attachment and faithful chromosome segregation during mitosis and meiosis. Dysregulation of CENPP has been implicated in chromosomal instability, a hallmark of many cancers and genetic disorders.
CENPP antibodies are valuable tools for studying centromere biology, cell cycle regulation, and mechanisms underlying aneuploidy. They are widely used in techniques like immunofluorescence, chromatin immunoprecipitation (ChIP), and Western blotting to localize CENPP within the centromere, assess its expression levels, or investigate its interactions with other centromeric components. These antibodies also aid in exploring links between centromere dysfunction and diseases, particularly cancers characterized by aberrant chromosome numbers. Recent studies have highlighted CENPP's potential as a biomarker for certain malignancies, driving interest in its clinical relevance. However, challenges remain in distinguishing cross-reactivity with other CCAN proteins, emphasizing the need for antibody validation in specific experimental systems.
×
