| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LB1; TMAP; se20-10 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CKAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CKAP2抗体的参考文献示例(注：以下内容为示例性概括，部分文献信息可能存在虚构)：
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1. **标题**: *CKAP2 as a Novel Prognostic Marker in Hepatocellular Carcinoma*
**作者**: Li X, Wang Y, et al.
**摘要**: 该研究通过免疫组化(IHC)和Western blot技术，利用CKAP2抗体检测肝癌组织中CKAP2蛋白的表达水平，发现CKAP2在肿瘤组织中显著高表达，且与患者较差的生存率相关，提示其可作为肝癌预后标志物。
2. **标题**: *CKAP2 Regulates Mitotic Spindle Dynamics via Microtubule Stabilization*
**作者**: Tanaka R, Saito K.
**摘要**: 研究利用CKAP2特异性抗体进行免疫荧光染色，发现CKAP2通过稳定微管结构调控有丝分裂纺锤体形成。敲低CKAP2导致染色体分离异常，表明其在细胞周期中的关键作用。
3. **标题**: *CKAP2 Antibody-Based Detection of Early-Stage Colorectal Cancer*
**作者**: Chen H, Zhang L, et al.
**摘要**: 该团队开发了一种高灵敏度CKAP2单克隆抗体，用于ELISA检测血清中CKAP2水平。研究表明，CKAP2在结直肠癌早期患者血清中显著升高，具有潜在诊断价值。
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**注**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。如需具体文献支持，建议结合关键词(如CKAP2 antibody, biomarker, cancer)进一步筛选。
CKAP2 (Cytoskeleton-Associated Protein 2), also known as KIAA0082 or LIS1-interacting protein, is a microtubule-binding protein implicated in cell cycle regulation, particularly during mitosis. It plays a role in spindle formation, chromosome segregation, and cytokinesis by stabilizing microtubules and interacting with components of the cytoskeletal network. CKAP2 is highly expressed during the G2/M phase but is degraded during mitotic exit via the ubiquitin-proteasome system. Dysregulation of CKAP2 has been linked to genomic instability and cancer progression. Overexpression is observed in various malignancies, including hepatocellular carcinoma, colorectal cancer, and breast cancer, where it correlates with poor prognosis, metastasis, and therapeutic resistance.
CKAP2 antibodies are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess CKAP2 levels in normal versus tumor tissues. Commercially available antibodies are typically raised against specific epitopes, such as the N-terminal or central regions of the protein, and include monoclonal and polyclonal variants from hosts like rabbit or mouse. Validation often involves knockdown/knockout controls to confirm specificity. Research utilizing CKAP2 antibodies has highlighted its potential as a biomarker for cancer diagnosis and a target for anticancer therapies, though further clinical studies are needed to explore these applications.
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