| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | OI9; CYPB; SCYLP; CYP-S1 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PPIB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPIB抗体的3篇虚构参考文献示例，内容基于常见研究方向概括：
1. **文献名称**: *Cyclophilin B (PPIB) Antibody Validation for Protein Folding Studies in Mammalian Cells*
**作者**: Smith A, et al.
**摘要**: 本研究验证了一种高特异性PPIB抗体在HeLa细胞中的应用，通过免疫沉淀和Western blot证实PPIB与内质网伴侣蛋白的相互作用，揭示其在胶原蛋白折叠中的关键作用，并发现PPIB缺失导致细胞外基质异常。
2. **文献名称**: *PPIB Overexpression as a Prognostic Marker in Glioblastoma: An Immunohistochemical Analysis*
**作者**: Chen L, et al.
**摘要**: 利用PPIB抗体对120例胶质母细胞瘤组织进行免疫组化分析，发现PPIB高表达与患者总生存期缩短显著相关，提示其可能作为潜在治疗靶点和预后标志物。
3. **文献名称**: *Comparative Evaluation of Commercial Anti-PPIB Antibodies in Neurodegenerative Disease Models*
**作者**: González R, et al.
**摘要**: 系统评估5种商品化PPIB抗体在小鼠阿尔茨海默病模型中的性能，发现抗体Clone 3F8在冰冻脑组织切片中表现最优，成功检测到tau蛋白异常聚集相关的PPIB表达上调。
(注：以上为模拟文献，实际引用需以真实出版物为准。)
**Background of PPIB Antibody**
The PPIB (Peptidyl-Prolyl cis-trans Isomerase B) antibody is a tool used to detect and study the PPIB protein, a member of the cyclophilin family of immunophilins. PPIB, also known as cyclophilin B, is a 22 kDa endoplasmic reticulum (ER)-resident protein that exhibits peptidyl-prolyl isomerase (PPIase) activity, facilitating proper protein folding by catalyzing the cis-trans isomerization of proline residues. It plays a critical role in collagen biosynthesis by interacting with prolyl hydroxylase and serving as a chaperone for procollagen.
PPIB is implicated in various physiological and pathological processes, including inflammation, viral replication, and cancer progression. Its overexpression has been observed in certain cancers, such as hepatocellular carcinoma and breast cancer, where it may promote tumor cell survival and metastasis. Additionally, PPIB interacts with the ER stress response pathway and modulates calcium signaling.
The PPIB antibody is widely utilized in research applications, including Western blotting, immunohistochemistry, and immunofluorescence, to investigate PPIB expression, localization, and function. It is also employed in studies exploring its role in diseases and potential therapeutic targeting. Commercially available PPIB antibodies are typically raised in rabbits or mice, validated for specificity against human, mouse, or rat PPIB isoforms. Understanding PPIB's molecular mechanisms through antibody-based assays contributes to insights into collagen-related disorders, cancer biology, and drug development.
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