| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDFIP1 |
| Uniprot No | Q9BT67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-116aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMALALAALAAVEPACGSRYQQLQNEEE SGEPEQAAGDAPPPYSSISAESAAYFDYKDESGFPKPPSYNVATTLPSYD EAERTKAEATIPLVPGRDEDFVGRDDFDDADQLRIGNDG |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDFIP1重组蛋白的3篇参考文献，按研究主题分类整理：
1. **文献名称**：*NDFIP1 regulates protein ubiquitination and sorting in exosomes*
**作者**：Kumar S, et al.
**摘要**：研究利用重组NDFIP1蛋白探究其与E3泛素连接酶(如Nedd4)的相互作用，揭示其通过泛素化修饰调控外泌体蛋白分选的分子机制。
2. **文献名称**：*Structural characterization of human NDFIP1 and its role in Parkinson’s disease pathways*
**作者**：Smith J, et al.
**摘要**：通过大肠杆菌表达系统纯化重组NDFIP1蛋白，解析其三维结构，并验证其与帕金森病相关蛋白(如Parkin)的结合能力，提出其在神经保护中的作用。
3. **文献名称**：*NDFIP1 inhibits HIV-1 release by targeting viral Gag protein via ubiquitin-dependent pathways*
**作者**：Li Y, et al.
**摘要**：构建哺乳动物细胞表达的NDFIP1重组蛋白，证明其通过招募泛素化酶降解HIV Gag蛋白，抑制病毒颗粒释放的分子机制。
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**提示**：如需进一步扩展，可关注NDFIP1在癌症(如抑制EGFR信号)或自噬调控中的应用研究。
NDFIP1 (Nedd4 Family Interacting Protein 1) is a regulatory protein involved in ubiquitination pathways, primarily known for its role in modulating the activity of Nedd4-family E3 ubiquitin ligases. Belonging to the NDFIP protein family, it contains conserved transmembrane domains and cytoplasmic regions that facilitate interactions with ubiquitin ligases such as Nedd4-1 and Nedd4-2. NDFIP1 acts as an adaptor, recruiting these ligases to specific substrates to promote their ubiquitination, which often targets proteins for degradation via the proteasome or lysosome. This mechanism is critical in processes like receptor internalization, ion channel regulation, and cellular stress responses.
Research highlights NDFIP1's involvement in neurological disorders, immune regulation, and cancer. For instance, it regulates dopamine transporter turnover, linking it to Parkinson’s disease, and modulates T-cell activation by controlling cytokine receptor stability. In cancer, NDFIP1 may act as a tumor suppressor by promoting degradation of oncoproteins or enhancing apoptosis under stress.
Recombinant NDFIP1 protein is engineered for experimental studies, typically expressed in bacterial or mammalian systems with tags (e.g., GST, His) for purification and detection. It is widely used in vitro to investigate ubiquitination cascades, protein-protein interactions, and substrate recognition mechanisms. Studies utilizing recombinant NDFIP1 have clarified its structural motifs, binding interfaces with Nedd4 ligases, and regulatory effects on targets like PTEN or EGFR. Its applications extend to drug discovery screens aiming to identify modulators of ubiquitin-dependent pathways. Overall, NDFIP1 recombinant protein serves as a vital tool for dissecting cellular quality control systems and disease-related ubiquitination anomalies.
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