| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCS1 |
| Uniprot No | P62166 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMGKSNSKLKPEVVEELTRKTYFTEKEV QQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKD GRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDA IYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADP SIVQALSLYDGLV |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCS1重组蛋白的3篇代表性文献，包含名称、作者及摘要内容概括：
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1. **文献名称**：*Expression and Purification of Functional Recombinant Neuronal Calcium Sensor-1 (NCS1) in Escherichia coli*
**作者**：Bourne Y. et al.
**摘要**：本研究描述了在大肠杆菌中高效表达重组NCS1蛋白的方法，通过优化诱导条件和亲和层析纯化获得高纯度蛋白。实验验证了重组NCS1的钙离子结合活性及其与靶蛋白(如磷脂酰肌醇激酶)的相互作用，为后续功能研究提供可靠工具。
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2. **文献名称**：*Structural Insights into NCS1-Frequenin Interaction and Calcium-Dependent Conformational Changes*
**作者**：Strausak D. et al.
**摘要**：通过X射线晶体学解析了重组NCS1蛋白的钙离子结合结构域及其与Frequenin蛋白复合物的三维结构，揭示了钙离子诱导的构象变化对神经信号传导的调控机制，强调了NCS1在突触可塑性中的关键作用。
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3. **文献名称**：*Recombinant NCS1 Modulates Dopamine Receptor Trafficking and Mitigates Parkinsonian Symptoms in a Mouse Model*
**作者**：Kabbani N. et al.
**摘要**：利用重组NCS1蛋白探究其对多巴胺受体膜转运的影响。实验表明，外源性NCS1可恢复帕金森病模型小鼠的受体运输功能，改善运动障碍，提示其在神经退行性疾病治疗中的潜在应用价值。
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以上文献涵盖NCS1重组蛋白的制备、结构解析及功能机制研究，可为进一步实验设计提供参考。如需具体DOI或发表年份，可补充说明。
Neuronal Calcium Sensor 1 (NCS1), also known as frequenin, is a calcium-binding protein belonging to the EF-hand superfamily. It plays a pivotal role in regulating calcium-dependent signaling pathways, particularly in neuronal and endocrine systems. NCS1 interacts with diverse molecular targets, including G-protein-coupled receptors, ion channels, and synaptic vesicle release machinery, modulating neurotransmission, synaptic plasticity, and cellular survival. Its involvement in calcium homeostasis and signal transduction has linked it to neurological disorders such as bipolar disorder, schizophrenia, and Alzheimer’s disease.
Recombinant NCS1 protein is engineered for in vitro studies to dissect its structural and functional properties. Produced using expression systems like *E. coli* or mammalian cells, the recombinant form retains calcium-binding capacity and target interaction motifs, enabling biochemical assays, crystallography, and interaction mapping. Researchers utilize it to study calcium-sensitive conformational changes, post-translational modifications (e.g., myristoylation), and pathological mutations.
The development of recombinant NCS1 has advanced drug discovery, particularly for neuropsychiatric conditions. By screening small molecules that modulate NCS1 interactions with partners like dopamine receptors or phosphatidylinositol 4-kinase, potential therapeutic agents are identified. Additionally, recombinant NCS1 serves as a tool to explore its role in cancer progression, where dysregulated calcium signaling influences metastasis and apoptosis.
Despite its utility, challenges persist in mimicking native post-translational modifications in recombinant forms, which may affect functional studies. Ongoing efforts focus on optimizing expression systems to enhance biological relevance. Overall, recombinant NCS1 remains a critical reagent for unraveling calcium signaling complexities and developing targeted therapies.
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