| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCK2 |
| Uniprot No | O43639 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTEEVIV IAKWDYTAQQ DQELDIKKNE RLWLLDDSKT WWRVRNAANR TGYVPSNYVE RKNSLKKGSL VKNLKDTLGL GKTRRKTSAR DASPTPSTDA EYPANGSGAD RIYDLNIPAF VKFAYVAERE DELSLVKGSR VTVMEKCSDG WWRGSYNGQI GWFPSNYVLE EVDEAAAESP SFLSLRKGAS LSNGQGSRVL HVVQTLYPFS SVTEEELNFE KGETMEVIEK PENDPEWWKC KNARGQVGLV PKNYVVVLSD GPALHPAHAP QISYTGPSSS GRFAGREWYY GNVTRHQAEC ALNERGVEGD FLIRDSESSP SDFSVSLKAS GKNKHFKVQL VDNVYCIGQR RFHTMDELVE HYKKAPIFTS EHGEKLYLVR ALQ |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCK2重组蛋白的3篇示例参考文献(虚构内容，仅供参考格式)：
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1. **文献名称**: *"Structural and Functional Analysis of Recombinant NCK2 Adaptor Protein in Cell Signaling"*
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功重组表达并纯化NCK2蛋白，通过X射线晶体学解析其三维结构，揭示其SH2和SH3结构域与下游效应分子(如PAK1)的相互作用机制，证实NCK2在调控细胞迁移中的关键作用。
2. **文献名称**: *"NCK2 Recombinant Protein Modulates Angiogenesis via VEGF Receptor Signaling Pathway"*
**作者**: Gupta S, et al.
**摘要**: 通过体外实验验证重组NCK2蛋白能够增强血管内皮细胞中VEGFR2的磷酸化水平，并促进血管生成。研究还发现NCK2的SH3结构域突变会显著抑制其促血管生成功能，提示其作为治疗靶点的潜力。
3. **文献名称**: *"Development of a High-Yield NCK2 Recombinant Protein Production System for Cancer Research"*
**作者**: Kim H, et al.
**摘要**: 本研究优化了哺乳动物细胞(HEK293)中NCK2重组蛋白的分泌表达条件，获得高纯度、高活性蛋白。实验证明该重组蛋白可特异性结合EGFR并影响肿瘤细胞的侵袭能力，为癌症信号通路研究提供工具。
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注：以上文献为示例性质，实际引用时需检索PubMed或Web of Science等数据库获取真实文献。
NCK2 (Non-catalytic region of tyrosine kinase adaptor protein 2) is a cytosolic adaptor protein involved in signal transduction pathways regulating cell migration, proliferation, and cytoskeletal dynamics. It belongs to the NCK family, which includes NCK1 and NCK2. sharing ～65% amino acid sequence homology. Structurally, NCK2 contains one SH2 domain and three SH3 domains, enabling interactions with phosphorylated tyrosine residues and proline-rich motifs in partner proteins, respectively. These interactions facilitate the assembly of multi-protein complexes that link cell surface receptors (e.g., receptor tyrosine kinases) to downstream effectors like RAS-MAPK, PAK, or WAVE regulatory pathways.
Recombinant NCK2 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) followed by chromatography. Its recombinant form is essential for *in vitro* studies to dissect signaling mechanisms, screen drug candidates, or analyze pathological mutations linked to diseases. Dysregulation of NCK2 has been implicated in cancer metastasis, neurodevelopmental disorders, and immune responses, making it a potential therapeutic target.
Research applications include pull-down assays to map protein interactions, kinase activity assays, and structural studies using X-ray crystallography or cryo-EM. Compared to endogenous NCK2. the recombinant version offers controlled purity and concentration, minimizing cross-reactivity in experiments. Recent studies highlight its role in neuronal axon guidance and T-cell receptor signaling, underscoring its biological versatility. Commercial availability of recombinant NCK2 accelerates functional genomics and drug discovery efforts aimed at modulating cytoskeletal or growth-related pathologies.
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