| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCALD |
| Uniprot No | P61601 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCALD(Neurocalcin delta)重组蛋白的假设性参考文献示例(仅供格式参考，具体文献需通过学术数据库验证)：
1. **文献名称**: "Reduction of NCALD by antisense oligonucleotides therapy as a strategy for spinal muscular atrophy"
**作者**: Y. Hua et al.
**摘要**: 研究通过下调NCALD表达增强脊髓性肌萎缩症(SMA)模型的运动神经元存活，利用重组NCALD蛋白验证其与钙信号通路的相互作用机制。
2. **文献名称**: "Expression and calcium-binding properties of recombinant human neurocalcin delta"
**作者**: S. Ozawa
**摘要**: 报道了人源NCALD重组蛋白在大肠杆菌中的表达与纯化，分析其钙离子依赖性构象变化及靶蛋白结合能力。
3. **文献名称**: "Neurocalcin delta modulates synaptic transmission via interaction with neuronal calcium sensors"
**作者**: H. Dawitz et al.
**摘要**: 利用重组NCALD蛋白研究其在神经元突触中的钙依赖性调控作用，揭示其通过抑制电压门控钙通道影响神经递质释放。
4. **文献名称**: "Structural insights into NCALD-mediated neuroprotection in Alzheimer's disease models"
**作者**: M. Rüppel et al.
**摘要**: 通过重组NCALD蛋白的体外实验，阐明其通过调控β-淀粉样蛋白毒性通路发挥神经保护作用的分子机制。
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**注意**：以上为假设案例，实际文献需通过PubMed/Google Scholar检索关键词如“NCALD recombinant protein”或“Neurocalcin delta expression”获取。推荐结合具体研究方向补充筛选(如疾病模型、结构生物学等)。
Neurocalcin delta (NCALD) is a calcium-binding protein belonging to the neuronal calcium sensor (NCS) family, which plays critical roles in mediating calcium-dependent signaling pathways. It is encoded by the *NCALD* gene and is highly conserved across vertebrates, with homologs identified in humans, mice, and other species. Structurally, NCALD contains three functional EF-hand motifs that bind Ca²⁺ ions, enabling it to act as a calcium sensor. Upon calcium binding, NCALD undergoes conformational changes, allowing it to interact with downstream targets and regulate cellular processes such as neurotransmitter release, ion channel activity, and synaptic plasticity.
Recombinant NCALD protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to ensure high purity and bioactivity. This engineered protein retains the native structure and calcium-binding properties of endogenous NCALD, making it a valuable tool for *in vitro* studies. Researchers utilize recombinant NCALD to investigate its interactions with partner proteins (e.g., G proteins, membrane receptors), its role in calcium signaling cascades, and its modulation of neuronal excitability. Notably, NCALD has been implicated in neurological disorders, including Alzheimer’s disease and Charcot-Marie-Tooth neuropathy, where dysregulated calcium homeostasis contributes to pathogenesis. Recombinant NCALD also aids in drug discovery, serving as a target for screening compounds that modulate calcium signaling. Its application extends to structural studies, such as X-ray crystallography or NMR, to resolve mechanisms underlying calcium-dependent activation. Overall, recombinant NCALD provides a versatile platform for deciphering calcium-mediated signaling in health and disease.
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