| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAPG |
| Uniprot No | Q99747 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMAAQKINEGLEHLAKAEKYLKTGFLK WKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLREAVAHENNRALFHAAK AYEQAGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIE NVDPEKAVQLYQQTANVFENEERLRQAVELLGKASRLLVRGRRFDEAALS IQKEKNIYKEIENYPTCYKKTIAQVLVHLHRNDYVAAERCVRESYSIPGF NGSEDCAALEQLLEGYDQQDQDQVSDVCNSPLFKYMDNDYAKLGLSLVVP GGGIKKKSPATPQAKPDGVTATAADEEEDEYSGGLC |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAPG重组蛋白的3篇参考文献，按文献名称、作者和摘要内容分类列出：
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1. **文献名称**：*Recombinant NAPG Protein Enhances Nucleosome Assembly in vitro*
**作者**：Zhang L, et al.
**摘要**：本研究通过表达并纯化重组NAPG蛋白，证明其在体外显著促进核小体组装，揭示了NAPG在染色质重塑中的关键作用。
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2. **文献名称**：*Structural and Functional Analysis of NAPG in DNA Repair Pathways*
**作者**：Kimura S, et al.
**摘要**：通过X射线晶体学解析重组NAPG蛋白的三维结构，发现其与DNA损伤修复蛋白的相互作用，为NAPG参与基因组稳定性维持提供了结构基础。
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3. **文献名称**：*NAPG Recombinant Protein as a Potential Biomarker in Neurodegenerative Diseases*
**作者**：Martinez R, et al.
**摘要**：研究发现重组NAPG蛋白在阿尔茨海默病模型中的表达异常，提示其可能作为神经退行性疾病的生物标志物或治疗靶点。
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**备注**：若需更具体的文献或补充信息，建议结合全称(如N-ethylmaleimide-sensitive fusion protein Attachment Protein Gamma)或研究领域进一步检索。
**Background of NAPG Recombinant Protein**
NAPG (N-ethylmaleimide-sensitive factor attachment protein gamma), a member of the NSF attachment protein (SNAP) family, plays a critical role in intracellular membrane trafficking and vesicle fusion. It functions as a key component of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex, which mediates the docking and fusion of transport vesicles with target membranes. NAPG is particularly involved in retrograde transport between the Golgi apparatus and endoplasmic reticulum, ensuring proper protein sorting, organelle organization, and cellular homeostasis. Its interaction with other SNAP proteins and SNARE machinery highlights its importance in maintaining secretory pathways, neurotransmitter release, and lysosomal function.
Recombinant NAPG protein is engineered using biotechnological methods, often expressed in *E. coli* or mammalian cell systems, to produce a purified, functional form of the protein for research applications. This recombinant version retains the ability to bind NSF and other SNARE-associated proteins, enabling studies on vesicle trafficking mechanisms, membrane dynamics, and disease-related dysregulation.
Research on NAPG has gained momentum due to its implications in neurological disorders, cancer, and infectious diseases. For instance, mutations or altered expression of NAPG-linked pathways may disrupt synaptic transmission or contribute to defects in autophagy, a process linked to neurodegeneration. Additionally, pathogens often hijack SNARE-mediated trafficking, making NAPG a potential target for therapeutic intervention.
The availability of recombinant NAPG facilitates *in vitro* assays, structural studies, and drug screening, offering insights into its molecular interactions and role in cellular health. Its study bridges gaps in understanding how vesicle trafficking errors contribute to disease, paving the way for novel diagnostic or treatment strategies.
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