| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NANOG |
| Uniprot No | Q9H9S0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-305aa |
| 氨基酸序列 | GSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTET VSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRT VFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKS KRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQT WNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQS CMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNM QPEDVESGGGGSPGRRRRRRRRRRR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NANOG重组蛋白的3篇参考文献及简要摘要：
1. **文献名称**：*"Production of Recombinant Human NANOG Protein in E. coli and Its Role in Maintaining Pluripotency"*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌表达系统成功纯化重组人NANOG蛋白，验证其通过激活OCT4/SOX2通路维持胚胎干细胞多能性的功能，为体外干细胞研究提供工具。
2. **文献名称**：*"Structural Analysis of NANOG Reveals a Dimerization-Dependent Transcriptional Activation Mechanism"*
**作者**：Li Y, et al.
**摘要**：通过晶体结构解析发现重组NANOG蛋白的二聚化对其转录激活功能至关重要，突变实验表明二聚化界面破坏会抑制多能性基因表达。
3. **文献名称**：*"Recombinant NANOG Protein Enhances Reprogramming Efficiency of Somatic Cells to iPSCs"*
**作者**：Wang X, et al.
**摘要**：证明在诱导多能干细胞(iPSCs)过程中添加重组NANOG蛋白可显著提高重编程效率，并降低对转录因子组合的依赖，为再生医学提供新策略。
(注：以上文献名为模拟概括，实际研究需参考具体论文数据库。)
**Background of NANOG Recombinant Protein**
NANOG is a transcription factor critical for maintaining pluripotency in embryonic stem cells (ESCs) and primordial germ cells. Named after the Celtic mythological land of eternal youth (*Tír na nÓg*), it plays a pivotal role in self-renewal by suppressing differentiation signals and sustaining the undifferentiated state. NANOG works in concert with other core pluripotency regulators, such as OCT4 and SOX2. forming a regulatory network that governs stem cell identity.
Recombinant NANOG protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems. The recombinant form retains the functional DNA-binding domain (homeodomain) and transcriptional activation properties of the native protein. Researchers utilize it to study mechanisms of pluripotency, cellular reprogramming (e.g., induced pluripotent stem cells), and regenerative medicine applications. It also aids in modeling developmental disorders or cancers linked to NANOG dysregulation.
Production involves codon optimization, vector design, and purification (e.g., affinity tags like His or GST). While bacterial systems offer high yield, mammalian systems may better preserve post-translational modifications critical for function. Variants include wild-type, tagged, or mutant forms for specific experimental needs.
Challenges include maintaining protein stability and activity *in vitro*, as NANOG’s function heavily depends on protein-protein/DNA interactions and cellular context. Despite this, recombinant NANOG remains a vital tool for advancing stem cell biology and therapeutic innovation.
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