| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAGK |
| Uniprot No | Q9UJ70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NAGK重组蛋白的代表性文献(注：文献信息为模拟生成，仅用于示例)：
1. **"Expression and Characterization of Recombinant N-Acetylglutamate Kinase from Arabidopsis thaliana"**
- 作者：Chen L. et al.
- 摘要：报道了拟南芥NAGK在大肠杆菌中的重组表达与纯化，分析了其酶动力学特性及精氨酸反馈抑制机制。
2. **"Crystal Structure of NAGK from Thermus thermophilus: Insights into Catalytic Mechanism"**
- 作者：Yamaguchi H. et al.
- 摘要：通过重组表达嗜热菌NAGK蛋白，解析了其晶体结构，揭示了ATP结合域和底物识别位点的关键氨基酸残基。
3. **"Functional Analysis of Human NAGK in Urea Cycle Disorders"**
- 作者：Wang Q. et al.
- 摘要：利用HEK293细胞表达人源重组NAGK，研究其突变体与尿素循环代谢疾病的关联，为临床诊断提供分子基础。
4. **"Optimized Production of NAGK in Pichia pastoris for Industrial Applications"**
- 作者：Rodríguez E. et al.
- 摘要：开发了毕赤酵母高效表达重组NAGK的工艺，验证其在酶法合成精氨酸前体化合物中的规模化应用潜力。
**提示**：实际文献需通过PubMed/SciHub等平台检索(关键词：NAGK + recombinant/prokaryotic expression/purification)，推荐结合UniProt数据库(ID: Q8T6A3)查找引用文献。
**Background of N-Acetylglutamate Kinase (NAGK) Recombinant Protein**
N-Acetylglutamate kinase (NAGK) is a critical enzyme in the arginine biosynthesis pathway, primarily found in microorganisms, plants, and some eukaryotes. It catalyzes the ATP-dependent phosphorylation of N-acetylglutamate (NAG) to form N-acetylglutamyl phosphate, a rate-limiting step in the production of arginine, an essential amino acid involved in protein synthesis, nitrogen metabolism, and nitric oxide signaling. NAGK’s activity is tightly regulated, often through feedback inhibition by arginine, highlighting its role in maintaining metabolic balance.
Recombinant NAGK proteins are engineered using genetic engineering techniques, where the *NAGK* gene is cloned into expression vectors (e.g., *E. coli*, yeast, or mammalian systems) to produce purified, functional enzyme variants. This approach enables large-scale production of NAGK with high purity and consistency, bypassing challenges associated with isolating the enzyme from native sources. Recombinant NAGK is widely utilized in biochemical and structural studies to investigate its catalytic mechanism, allosteric regulation, and interactions with substrates or inhibitors.
Structurally, NAGK belongs to the amino acid kinase family, characterized by a conserved ATP-binding domain and a substrate-specific catalytic site. Recent crystallographic and cryo-EM studies have resolved its 3D architecture, revealing dynamic conformational changes during catalysis and regulation. These insights have spurred applications in drug discovery (e.g., targeting microbial NAGK for antibiotic development) and agricultural biotechnology (e.g., enhancing nitrogen use efficiency in crops). Additionally, recombinant NAGK serves as a tool enzyme in metabolic engineering and synthetic biology to optimize arginine pathways for industrial or therapeutic purposes.
Overall, recombinant NAGK represents a pivotal resource for advancing research in enzymology, metabolic diseases, and biotechnological innovations.
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