| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAP1L1 |
| Uniprot No | P55209 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MADIDNKEQS ELDQDLDDVE EVEEEETGEE TKLKARQLTV QMMQNPQILA ALQERLDGLV ETPTGYIESL PRVVKRRVNA LKNLQVKCAQ IEAKFYEEVH DLERKYAVLY QPLFDKRFEI INAIYEPTEE ECEWKPDEED EISEELKEKA KIEDEKKDEE KEDPKGIPEF WLTVFKNVDL LSDMVQEHDE PILKHLKDIK VKFSDAGQPM SFVLEFHFEP NEYFTNEVLT KTYRMRSEPD DSDPFSFDGP EIMGCTGCQI DWKKGKNVTL KTIKKKQKHK GRGTVRTVTK TVSNDSFFNF FAPPEVPESG DLDDDAEAIL AADFEIGHFL RERIIPRSVL YFTGEAIEDD DDDYDEEGEE ADEEGEEEGD EENDPDYDPK KDQNPAEC |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NAP1L1重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: *"Expression and purification of recombinant human NAP1L1 from Escherichia coli for structural and functional studies"*
**作者**: Zhang Y, et al.
**摘要**: 该研究报道了人源NAP1L1基因在大肠杆菌中的高效重组表达及纯化策略，通过优化诱导条件和亲和层析技术获得高纯度蛋白。重组蛋白被用于体外染色质组装实验，证实其促进组蛋白H2A/H2B二聚体与DNA结合的分子功能。
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2. **文献名称**: *"Crystal structure of NAP1L1 reveals a conserved histone chaperone domain essential for chromatin remodeling"*
**作者**: Watanabe S, et al.
**摘要**: 本文解析了重组NAP1L1蛋白的晶体结构，鉴定出其核心的组蛋白伴侣功能域，并通过突变实验证明该区域对组蛋白结合和染色质动态调控至关重要。研究为理解NAP1L1在表观遗传调控中的作用提供了结构基础。
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3. **文献名称**: *"Recombinant NAP1L1 interacts with DNA methyltransferase DNMT3A and modulates its enzymatic activity in vitro"*
**作者**: Li H, et al.
**摘要**: 通过重组NAP1L1蛋白与DNMT3A的体外结合实验，研究发现NAP1L1能增强DNMT3A的DNA甲基转移酶活性，提示其在表观遗传修饰中的协同作用机制，可能影响基因沉默和细胞分化过程。
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注：上述文献信息为模拟示例，实际引用时需核实具体文献来源及准确性。建议通过PubMed、Google Scholar等平台以关键词“NAP1L1 recombinant”或“NAP1L1 expression”检索最新研究。
NAP1L1 (Nucleosome Assembly Protein 1-Like 1) is a conserved eukaryotic protein belonging to the nucleosome assembly protein (NAP) family, which plays critical roles in chromatin remodeling, histone chaperoning, and epigenetic regulation. It facilitates the assembly and disassembly of nucleosomes by interacting with histones H3 and H4. thereby influencing DNA replication, transcription, and repair. NAP1L1 is ubiquitously expressed, with elevated levels observed in proliferating cells, suggesting its involvement in cell cycle progression and differentiation. Dysregulation of NAP1L1 has been implicated in cancers, neurodevelopmental disorders, and viral infections, highlighting its biological significance.
Recombinant NAP1L1 protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), retains the functional domains necessary for histone binding and nucleosome dynamics. Structural studies reveal a characteristic acidic region critical for histone interactions and a conserved NAP domain mediating oligomerization. Researchers utilize recombinant NAP1L1 to dissect its molecular mechanisms in vitro, including chromatin reconstitution assays, histone exchange studies, and protein interaction analyses. Its applications extend to drug discovery, particularly in targeting chromatin-related pathways in oncology, and as a tool to explore epigenetic perturbations in disease models. Recent advances in cryo-EM and biophysical techniques have further enabled detailed characterization of its conformational dynamics. Ongoing research aims to clarify its tissue-specific roles and therapeutic potential in chromatin-associated pathologies.
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