| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAP1L4 |
| Uniprot No | Q99733 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADHSFS DGVPSDSVEA AKNASNTEKL TDQVMQNPRV LAALQERLDN VPHTPSSYIE TLPKAVKRRI NALKQLQVRC AHIEAKFYEE VHDLERKYAA LYQPLFDKRR EFITGDVEPT DAESEWHSEN EEEEKLAGDM KSKVVVTEKA AATAEEPDPK GIPEFWFTIF RNVDMLSELV QEYDEPILKH LQDIKVKFSD PGQPMSFVLE FHFEPNDYFT NSVLTKTYKM KSEPDKADPF SFEGPEIVDC DGCTIDWKKG KNVTVKTIKK KQKHKGRGTV RTITKQVPNE SFFNFFNPLK ASGDGESLDE DSEFTLASDF EIGHFFRERI VPRAVLYFTG EAIEDDDNFE EGEEGEEEEL EGDEEGEDED DAEINPKV |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAP1L4重组蛋白的模拟参考文献示例(基于公开领域知识概括，建议通过PubMed或SciHub获取真实文献)：
1. **《NAP1L4 promotes hepatocellular carcinoma progression through recruiting HDAC1 to suppress p53 transcription》**
- 作者：Li X, et al.
- 摘要：研究揭示了NAP1L4重组蛋白在肝癌中的作用机制，通过招募组蛋白去乙酰化酶HDAC1抑制p53转录活性，促进肿瘤细胞增殖和转移。
2. **《Structural characterization of recombinant human NAP1L4 and its nucleosome assembly activity》**
- 作者：Zhang Y, et al.
- 摘要：利用大肠杆菌表达系统获得高纯度重组人NAP1L4蛋白，通过X射线晶体学解析其结构，并证明其在体外染色质组装中的关键作用。
3. **《NAP1L4 interacts with reprogramming factors to modulate pluripotency in embryonic stem cells》**
- 作者：Wang H, et al.
- 摘要：研究发现重组NAP1L4蛋白与Oct4/Sox2等重编程因子相互作用，通过表观遗传调控维持胚胎干细胞多能性，CRISPR敲除实验显示其缺失导致分化异常。
4. **《Dysregulation of NAP1L4 in neurodevelopmental disorders: Insights from recombinant protein-based interactome analysis》**
- 作者：Chen R, et al.
- 摘要：通过重组NAP1L4蛋白的互作组学分析，发现其与多种神经发育相关蛋白(如MECP2)存在物理结合，提示其在自闭症谱系障碍中的潜在病理机制。
**注**：以上为基于领域知识的模拟摘要，实际文献请通过PubMed、Google Scholar或Web of Science以"NAP1L4 recombinant"为关键词检索。
NAP1L4 (Nucleosome Assembly Protein 1 Like 4) is a member of the NAP1 family of histone chaperones, which play critical roles in nucleosome assembly, chromatin remodeling, and epigenetic regulation. This protein is evolutionarily conserved and shares structural homology with other NAP1 family members, characterized by conserved NAP1 domains that mediate interactions with histones and chromatin-modifying complexes. NAP1L4 is primarily involved in facilitating the deposition of histones H2A and H2B during DNA replication and repair, ensuring proper nucleosome organization and genome stability. It also participates in transcriptional regulation by modulating chromatin accessibility for transcription factors and RNA polymerase.
Recombinant NAP1L4 protein is engineered through molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its production enables functional studies, including in vitro chromatin reconstitution assays, protein interaction analyses, and investigations into its role in cell cycle progression, differentiation, and development. Dysregulation of NAP1L4 has been linked to several pathologies. For instance, aberrant expression is observed in cancers such as hepatocellular carcinoma and neuroblastoma, where it may influence tumor proliferation or metastasis. Additionally, studies suggest its involvement in neurodevelopmental disorders, potentially through disrupted chromatin dynamics during neural differentiation.
Research on recombinant NAP1L4 also explores its interplay with viral pathogens, as some viruses exploit histone chaperones to manipulate host chromatin for replication. Overall, NAP1L4 serves as a valuable tool for dissecting chromatin biology and disease mechanisms, with implications for developing targeted epigenetic therapies.
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