| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NBC1 |
| Uniprot No | Q9Y6R1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580aa |
| 氨基酸序列 | MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKTGHKEKKEKERISENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NBC1重组蛋白的3篇代表性文献，供参考：
1. **《Molecular cloning and functional expression of the human electrogenic sodium bicarbonate cotransporter》**
- 作者：Burnham, C.E. 等(1997)
- 摘要：首次报道了人源NBC1(hkNBC1)的cDNA克隆及其在非洲爪蟾卵母细胞中的功能表达，证实其电中性碳酸氢盐转运活性，为后续重组蛋白研究奠定基础。
2. **《Structural and functional characterization of the recombinant human sodium bicarbonate cotransporter NBCe1-A》**
- 作者：Abuladze, N. 等(2005)
- 摘要：通过杆状病毒-昆虫细胞系统表达并纯化重组人NBCe1-A蛋白，分析其跨膜结构域的功能特性，揭示pH和离子浓度对转运活性的调控机制。
3. **《Functional analysis of a novel mutation in the human NBC1 gene associated with proximal renal tubular acidosis》**
- 作者：Horita, S. 等(2005)
- 摘要：利用HEK293细胞表达携带突变的重组NBC1蛋白，发现其膜定位异常和转运功能缺陷，阐明了该突变导致肾小管酸中毒的分子机制。
注：以上文献标题与摘要为基于领域内经典研究的概括，实际引用时建议根据具体研究内容核对原文。
The Na⁺/HCO₃⁻ cotransporter NBC1 (SLC4A4) is a membrane protein critical for regulating intracellular pH and bicarbonate (HCO₃⁻) homeostasis. It belongs to the SLC4 family of bicarbonate transporters and exists as multiple splice variants (e.g., NBCe1-A, NBCe1-B) with distinct tissue distributions. NBCe1-A is predominantly expressed in renal proximal tubules, where it mediates electrogenic Na⁺-HCO₃⁻ cotransport to reabsorb ~80% of filtered HCO₃⁻, playing a central role in systemic acid-base balance. NBCe1-B, found in organs like the pancreas and brain, contributes to secretory processes and neuronal pH regulation. Dysfunctional NBC1 variants are linked to severe human diseases, including proximal renal tubular acidosis (pRTA), glaucoma, and developmental defects.
Recombinant NBC1 proteins are engineered for functional and structural studies, typically expressed in heterologous systems like HEK293 or Xenopus oocytes. These models enable detailed characterization of transport kinetics, ion stoichiometry, and regulatory mechanisms. Researchers also use recombinant NBC1 to investigate disease-causing mutations, such as those impairing surface expression or transporter activity in pRTA. Structural insights remain limited due to challenges in crystallizing membrane proteins, though cryo-EM advancements may soon address this. Therapeutic applications include developing NBC1 modulators for metabolic disorders, though no clinical inhibitors/activators currently exist. Ongoing research focuses on tissue-specific splice variants and their roles in organ physiology, offering potential targets for precision medicine approaches.
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