| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NARS |
| Uniprot No | O43776 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMVLAELYVSDREGSDATGDGTKEKPFK TGLKALMTVGKEPFPTIYVDSQKENERWNVISKSQLKNIKKMWHREQMKS ESREKKEAEDSLRREKNLEEAKKITIKNDPSLPEPKCVKIGALEGYRGQR VKVFGWVHRLRRQGKNLMFLVLRDGTGYLQCVLADELCQCYNGVLLSTES SVAVYGMLNLTPKGKQAPGGHELSCDFWELIGLAPAGGADNLINEESDVD VQLNNRHMMIRGENMSKILKARSMVTRCFRDHFFDRGYYEVTPPTLVQTQ VEGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSR TRRHLAEYTHVEAECPFLTFDDLLNRLEDLVCDVVDRILKSPAGSIVHEL NPNFQPPKRPFKRMNYSDAIVWLKEHDVKKEDGTFYEFGEDIPEAPERLM TDTINEPILLCRFPVEIKSFYMQRCPEDSRLTESVDVLMPNVGEIVGGSM RIFDSEEILAGYKREGIDPTPYYWYTDQRKYGTCPHGGYGLGLERFLTWI LNRYHIRDVCLYPRFVQRCTP |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NARS(天冬酰胺酰-tRNA合成酶)重组蛋白的假设参考文献示例，供参考：
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1. **文献名称**：*Cloning and Expression of Recombinant Human Asparaginyl-tRNA Synthetase in E. coli*
**作者**：Zhang, Y. et al.
**摘要**：本研究成功克隆了人源NARS基因，并在大肠杆菌中实现高效可溶性表达。通过优化诱导条件，获得高纯度重组蛋白，并验证其催化活性，为后续酶学及结构研究奠定基础。
2. **文献名称**：*Structural Insights into the Catalytic Mechanism of NARS through X-ray Crystallography*
**作者**：Lee, S. & Kim, J.
**摘要**：解析了重组NARS蛋白的晶体结构(分辨率2.1Å)，揭示了其底物结合域和ATP结合位点的构象变化，阐明了天冬酰胺-tRNA合成过程中的关键催化残基。
3. **文献名称**：*Dysregulation of NARS in Pancreatic Cancer: Implications for Therapeutic Targeting*
**作者**：Wang, H. et al.
**摘要**：发现胰腺癌组织中NARS表达显著上调，重组蛋白实验表明其通过调控mTOR通路促进肿瘤增殖。提示NARS或成为潜在治疗靶点。
4. **文献名称**：*Engineering Thermostable NARS for Industrial Applications*
**作者**：Müller, R. & Schmidt, A.
**摘要**：通过定向进化技术改造重组NARS，获得耐高温突变体(65℃下活性保持90%)，显著提升其在体外蛋白质合成系统中的稳定性与效率。
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**注**：以上为模拟参考文献，实际文献需通过PubMed、Web of Science等平台检索关键词(如“Asparaginyl-tRNA Synthetase recombinant”、“NARS expression”)获取。
**Background of NARS Recombinant Protein**
NARS (Asparaginyl-tRNA Synthetase) is a member of the aminoacyl-tRNA synthetase (aaRS) family, enzymes essential for protein biosynthesis. These enzymes catalyze the attachment of specific amino acids to their corresponding transfer RNAs (tRNAs), ensuring the accuracy of genetic code translation. NARS specifically links the amino acid asparagine (Asn) to its cognate tRNA, a critical step in ribosomal protein synthesis. Dysregulation or mutations in NARS have been implicated in neurodevelopmental disorders, mitochondrial dysfunction, and autoimmune diseases, highlighting its biological and medical significance.
The development of **recombinant NARS protein** leverages genetic engineering to produce purified, functional enzyme variants in heterologous systems (e.g., *E. coli*, yeast, or mammalian cells*). Recombinant technology allows large-scale production, structural modification, and functional characterization of NARS, enabling studies on its catalytic mechanisms, substrate specificity, and interactions with tRNA or regulatory proteins.
In research, recombinant NARS is pivotal for structural biology (e.g., X-ray crystallography, cryo-EM) to resolve 3D architectures and active-site dynamics. It also serves as a tool in drug discovery, particularly for targeting aaRS enzymes in pathogens (e.g., antibiotics) or correcting defects in human NARS-linked diseases. Additionally, NARS autoantibodies are biomarkers in autoimmune conditions like antisynthetase syndrome, driving demand for recombinant NARS in diagnostic assays.
Advances in synthetic biology further exploit engineered NARS variants to expand the genetic code, incorporating non-canonical amino acids into proteins for biotechnological or therapeutic applications. Overall, recombinant NARS bridges fundamental biochemistry with translational innovation, offering insights into cellular biology and avenues for therapeutic intervention.
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