| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCF1 |
| Uniprot No | P14598 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEF HKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCST LMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTAT DITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKA KRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEG EAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPP RRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPL EEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAVVEHHHHHH |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCF1重组蛋白的3篇示例参考文献(虚构示例，仅作格式参考)：
1. **文献名称**：*Functional Restoration of NADPH Oxidase in Chronic Granulomatous Disease by Recombinant NCF1 Protein*
**作者**：D. Roos et al.
**摘要**：研究通过体外表达重组NCF1蛋白，验证其与p22-phox亚基的结合能力，证明其在慢性肉芽肿病(CGD)患者细胞中恢复NADPH氧化酶活性氧(ROS)产生的潜力。
2. **文献名称**：*Gene Therapy Using Recombinant NCF1 for p47-phox-Deficient Neutrophils*
**作者**：P.G. Heyworth et al.
**摘要**：利用腺病毒载体递送重组NCF1基因至缺陷型中性粒细胞，成功修复氧化酶复合物功能，为CGD的基因治疗提供实验依据。
3. **文献名称**：*Structural and Functional Analysis of NCF1 Domains in ROS Generation*
**作者**：A.W. Segal et al.
**摘要**：通过重组NCF1蛋白的截断体实验，揭示其SH3结构域在结合p67-phox和激活NADPH氧化酶中的关键作用，为靶向药物设计提供基础。
**注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“recombinant NCF1 protein”或“NCF1 NADPH oxidase”获取。
**Background of NCF1 Recombinant Protein**
NCF1 (Neutrophil Cytosolic Factor 1), also known as p47phox, is a critical subunit of the NADPH oxidase complex, a key enzyme system in innate immunity. This multiprotein complex generates reactive oxygen species (ROS), such as superoxide anions, to combat microbial pathogens. NCF1 acts as an organizer, facilitating the assembly of NADPH oxidase by interacting with other subunits (e.g., p22phox, p67phox, and Rac) upon cellular activation. Its structure includes SH3 domains that bind proline-rich motifs in partner proteins, enabling signal transduction during the "oxidative burst" in phagocytes.
Mutations in the *NCF1* gene are linked to chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by defective ROS production, leading to recurrent infections and granuloma formation. Beyond immunity, dysregulated NCF1/NADPH oxidase activity is implicated in inflammatory diseases (e.g., rheumatoid arthritis) and oxidative stress-related conditions.
Recombinant NCF1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It retains the ability to reconstitute NADPH oxidase activity in vitro, making it valuable for dissecting molecular mechanisms of oxidase assembly, screening therapeutic agents for CGD, or studying ROS-related signaling pathways. Researchers also utilize NCF1 recombinant proteins to develop diagnostic tools or explore its role in autoimmune and inflammatory models. Its applications extend to gene therapy research, where functional NCF1 delivery aims to correct oxidase defects in CGD patients.
Overall, NCF1 recombinant protein serves as a vital tool in immunology and biomedical research, bridging mechanistic insights with therapeutic innovation.
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