| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCBP2 |
| Uniprot No | P52298 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCBP2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structural basis of mRNA cap recognition by the heterodimeric nuclear cap-binding complex*
**作者**：Mazza, C. et al.
**摘要**：该研究解析了人源核帽结合复合物(CBC)的结构，其中NCBP2与CBP80形成异源二聚体。通过重组表达纯化的NCBP2/CBP80蛋白，揭示了其识别mRNA 5'端帽结构的分子机制，为mRNA加工机制提供了结构基础。
2. **文献名称**：*NCBP2 modulates neurodevelopment via a novel RNA-binding function in addition to canonical CBC activity*
**作者**：Gonatopoulos-Pournatzis, T. et al.
**摘要**：研究利用重组NCBP2蛋白进行体外RNA结合实验，发现NCBP2不仅参与帽依赖性mRNA转运，还具有独立于CBC复合物的RNA结合功能，影响神经元分化相关基因的剪接调控。
3. **文献名称**：*Recombinant NCBP2 facilitates high-throughput screening for inhibitors of viral cap-snatching mechanisms*
**作者**：Ishimura, R. et al.
**摘要**：开发了基于重组NCBP2蛋白的体外筛选平台，用于鉴定抑制流感病毒劫持宿主mRNA帽结构的化合物。研究证实重组NCBP2保留了与病毒聚合酶的相互作用能力，为抗病毒药物开发提供工具。
(注：上述文献为示例性概括，实际研究需参考具体论文。)
**Background of NCBP2 Recombinant Protein**
NCBP2 (Nuclear Cap-Binding Protein Subunit 2) is a critical component of the nuclear cap-binding complex (CBC), which plays a central role in RNA processing and transport. The CBC, composed of NCBP2 and its partner NCBP1. binds to the 7-methylguanosine (m⁷G) cap structure at the 5′ end of nascent RNA polymerase II-transcribed RNAs, including pre-mRNAs and certain non-coding RNAs. This interaction is essential for coordinating multiple steps in gene expression, such as splicing, polyadenylation, nuclear export, and RNA stability. NCBP2 also participates in alternative splicing regulation and links transcription to downstream RNA surveillance mechanisms.
Structurally, NCBP2 contains a conserved RNA-binding domain and interacts with NCBP1 to form a heterodimeric complex. Dysregulation of NCBP2 has been implicated in various diseases, including cancers and viral infections, due to its role in modulating RNA metabolism and immune responses. For instance, some viruses exploit the CBC to promote viral RNA stability and translation, while NCBP2 mutations or altered expression may contribute to oncogenesis.
Recombinant NCBP2 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce highly pure, functional protein for *in vitro* studies. It serves as a vital tool for investigating cap-binding mechanisms, RNA-protein interactions, and CBC-dependent cellular pathways. Researchers utilize NCBP2 recombinant protein in assays like pull-down experiments, structural studies (e.g., X-ray crystallography), and functional analyses to dissect its role in RNA biology and disease. Its applications extend to drug discovery, particularly in targeting RNA-processing pathways implicated in cancer or viral replication.
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