| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDRG3 |
| Uniprot No | Q9UGV2-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDELQDV QLTEIKPLLN DKEHDIETTH GVVHVTIRGL PKGNRPVILT YHDIGLNHKS CFNAFFNFED MQEITQHFAV CHVDAPGQQE GAPSFPTGYQ YPTMDELAEM LPPVLTHLSL KSIIGIGVGA GAYILSRFAL NHPELVEGLV LINVDPCAKG WIDWAASKLS GLTTNVVDII LAHHFGQEEL QANLDLIQTY RMHIAQDINQ DNLQLFLNSY NGRRDLEIER PILGQNDNKS KTLKCSTLLV VGDNSPAVEA VVECNSRLNP INTTLLKMAD CGGLPQVVQP GKLTEAFKYF LQGMGYIPSA SMTRLARSRT HSTSSSLGSG ESPFSRSVTS NQSDGTQESC ESPDVLDRHQ TMEVSC |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDRG3重组蛋白的3篇参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*"NDRG3 modulates hypoxia tolerance through regulation of the mitochondrial electron transport chain"*
**作者**：Lee JH, et al.
**摘要**：本研究通过重组表达人源NDRG3蛋白，揭示其在低氧环境下通过调节线粒体电子传递链复合物I的稳定性，增强细胞对缺氧的适应性。实验表明，重组NDRG3可抑制活性氧(ROS)积累，为肿瘤细胞在缺氧微环境中的存活提供机制支持。
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2. **文献名称**：*"Expression, purification, and functional characterization of recombinant NDRG3 in prostate cancer cells"*
**作者**：Zhang Y, et al.
**摘要**：作者利用大肠杆菌系统成功表达并纯化重组NDRG3蛋白，发现其在前列腺癌细胞中通过激活Akt/mTOR信号通路促进细胞增殖和迁移。研究为NDRG3在癌症进展中的作用提供了分子水平的实验依据。
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3. **文献名称**：*"Structural insights into the pH-dependent activation of NDRG3 in cellular stress response"*
**作者**：Wang Q, et al.
**摘要**：该研究解析了重组NDRG3蛋白的晶体结构，发现其C端结构域在酸性条件下发生构象变化，从而触发与分子伴侣HSP90的相互作用。这一机制解释了NDRG3在细胞应激(如营养缺乏或酸化)中的动态调控功能。
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**备注**：以上文献信息为模拟示例，具体内容需根据实际发表论文调整。建议通过PubMed或Web of Science以“NDRG3 recombinant protein”为关键词检索最新研究。
**Background of NDRG3 Recombinant Protein**
NDRG3 (N-Myc downstream-regulated gene 3) is a member of the NDRG family, which comprises four conserved proteins (NDRG1–4) implicated in diverse cellular processes, including differentiation, stress response, and carcinogenesis. Unlike other family members, NDRG3 is less characterized but shares structural features such as an α/β hydrolase-fold domain, though its enzymatic function remains unclear. It is ubiquitously expressed, with higher levels in the brain, liver, and reproductive organs, suggesting tissue-specific roles.
Recombinant NDRG3 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced using bacterial (e.g., *E. coli*) or mammalian expression systems, it retains functional epitopes for interaction assays, antibody development, or structural analysis. Post-translational modifications, like phosphorylation, can be preserved in eukaryotic systems to mimic native protein behavior.
Functionally, NDRG3 is linked to hypoxia adaptation, cell proliferation, and metabolism. It interacts with signaling pathways, including HIF-1α and androgen receptor pathways, and may act as a stress-responsive mediator. In cancer, NDRG3 exhibits dual roles: it suppresses tumor growth in some contexts (e.g., prostate cancer) but promotes metastasis in others (e.g., hepatocellular carcinoma), highlighting its context-dependent regulation.
Research on recombinant NDRG3 aids in clarifying its role in diseases and exploring therapeutic targeting. However, its precise mechanisms, including substrate binding or enzymatic activity, remain open questions, necessitating further biochemical and functional studies.
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