| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | EB3; RP3; EBF3; EBF3-S |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAPRE3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAPRE3抗体的3篇参考文献示例(注：内容为模拟生成，具体文献需根据实际数据库查询)：
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1. **文献名称**：*"Characterization of MAPRE3 Antibody Specificity in Microtubule Plus-End Tracking"*
**作者**：Smith J, et al.
**摘要**：本研究验证了一种新型MAPRE3多克隆抗体的特异性，通过免疫印迹和免疫荧光证实其能特异性识别内源性MAPRE3蛋白，并揭示其在HeLa细胞中对微管正端动态的调控作用。
2. **文献名称**：*"EB Family Protein MAPRE3 Interaction with APC in Colorectal Cancer Models"*
**作者**：Lee H, et al.
**摘要**：利用MAPRE3抗体探究其与腺瘤性息肉病(APC)蛋白的相互作用，发现MAPRE3在结直肠癌细胞中通过稳定微管网络促进肿瘤迁移，抗体阻断实验进一步支持其功能重要性。
3. **文献名称**：*"Development of a Monoclonal Antibody for MAPRE3 and Its Application in Neurodegenerative Disease"*
**作者**：Wang Y, et al.
**摘要**：报道了一种高亲和力MAPRE3单克隆抗体的开发，并在阿尔茨海默病模型中证实MAPRE3表达异常与微管稳定性受损相关，抗体可用于脑组织切片中的病理检测。
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建议通过PubMed或Google Scholar检索实际文献，使用关键词“MAPRE3 antibody”、“MAPRE3 immunological study”等，并筛选涉及抗体开发、验证或功能研究的文章。
The MAPRE3 antibody targets the microtubule-associated protein RP/EB family member 3 (MAPRE3), also known as EB3. a critical regulator of microtubule dynamics in eukaryotic cells. EB3 belongs to the end-binding (EB) protein family, which includes EB1. EB2. and EB3. and is characterized by its ability to bind the plus ends of growing microtubules. These proteins facilitate interactions between microtubules and cellular components, playing essential roles in cell division, intracellular transport, cell migration, and polarization. EB3 specifically contributes to microtubule stabilization, actin-microtubule crosstalk, and spindle orientation during mitosis. It is widely expressed in tissues, particularly in the brain, where it supports neuronal development and synaptic plasticity.
The MAPRE3 antibody is a key tool for studying EB3 localization, expression, and function. It is used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to investigate EB3’s role in cellular processes and diseases. Dysregulation of EB3 has been linked to cancer progression, neurological disorders, and developmental defects. For example, EB3 overexpression in tumors correlates with metastasis and poor prognosis, while its deficiency impacts neuronal migration. Researchers also employ MAPRE3 antibodies to explore EB3’s interaction with binding partners, such as APC (adenomatous polyposis coli) and CLIP-170. which mediate microtubule-cortex anchoring. Available as monoclonal or polyclonal variants, these antibodies are validated for specificity across human, mouse, and rat models, aiding mechanistic studies in cell biology and disease research.
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