| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GAB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GAB2抗体的3篇参考文献及其摘要概括：
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1. **"GAB2 regulates the migratory behaviors and E-cadherin expression via activation of the PI3K/Akt signaling pathway in breast cancer cells"**
- **作者**: Ke Y, et al.
- **摘要**: 本研究利用GAB2特异性抗体，通过Western blot和免疫荧光技术，揭示了GAB2通过激活PI3K/Akt信号通路促进乳腺癌细胞迁移，并调控E-钙黏蛋白表达的作用机制。
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2. **"Role of the adapter protein GAB2 in beta-catenin signaling"**
- **作者**: Sattler M, et al.
- **摘要**: 通过免疫共沉淀和GAB2抗体检测，研究发现GAB2与β-catenin相互作用，增强Wnt信号通路活性，并参与肿瘤细胞的增殖和侵袭过程。
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3. **"Grb2-associated binder 2 (GAB2) is a novel regulator of allergic mast cell signaling"**
- **作者**: Mao Y, et al.
- **摘要**: 该文献使用GAB2抗体进行免疫组化分析，发现GAB2在肥大细胞中高表达，并通过调控IgE受体信号通路，影响过敏反应中组胺释放等关键过程。
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(注：以上文献信息为示例，实际引用时建议通过PubMed或学术数据库核对具体细节。)
The GAB2 (GRB2-associated binding protein 2) antibody is a key tool for studying the role of GAB2. an adaptor protein involved in signal transduction pathways regulating cell growth, differentiation, and survival. GAB2 lacks enzymatic activity but serves as a scaffolding molecule, interacting with receptors, kinases, and other signaling proteins through its structural domains, including an N-terminal pleckstrin homology (PH) domain and multiple binding sites for SH3 and SH2 domain-containing proteins. It is notably recruited by GRB2 (Growth Factor Receptor-Bound Protein 2) to activate downstream pathways such as PI3K/AKT and RAS/MAPK, which are critical in cellular responses to growth factors, cytokines, and extracellular stimuli.
Dysregulation of GAB2 has been linked to various cancers, including breast cancer, leukemia, and melanoma, where it promotes oncogenic signaling, metastasis, and therapy resistance. For example, GAB2 amplification or overexpression enhances PI3K signaling in HER2-positive breast cancer, while in hematological malignancies, it interacts with BCR-ABL or FLT3 mutants to drive proliferation. GAB2 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, phosphorylation status, and subcellular localization in research models or clinical samples. These studies help elucidate GAB2's pathological mechanisms and its potential as a therapeutic target. Additionally, phospho-specific GAB2 antibodies aid in analyzing activation-dependent signaling events, further advancing drug discovery and biomarker development.
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