Rabbit Polyclonal NR3C1 Antibody

The NR3C1 antibody targets the glucocorticoid receptor (GR), encoded by the NR3C1 (Nuclear Receptor Subfamily 3 Group C Member 1) gene. GR is a ligand-activated transcription factor critical for mediating the effects of glucocorticoids, which regulate stress response, immune function, metabolism, and inflammation. Upon binding cortisol (or synthetic analogs like dexamethasone), GR translocates to the nucleus, where it modulates gene expression by interacting with glucocorticoid response elements (GREs) or tethering to other transcription factors. NR3C1 antibodies are widely used in research to study GR expression, localization, and function in tissues and cell lines. They are essential tools for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Dysregulation of GR signaling is linked to diseases like Cushing's syndrome, autoimmune disorders, depression, and cancer, making these antibodies valuable for mechanistic and clinical studies. Commercially available NR3C1 antibodies are raised against epitopes in the N-terminal, DNA-binding, or ligand-binding domains, with specificity validated via knockout controls. Researchers must consider species reactivity (human, mouse, rat) and post-translational modifications (e.g., phosphorylation, ubiquitination) when selecting antibodies. Reliable NR3C1 detection aids in exploring glucocorticoid resistance mechanisms or tissue-specific GR dynamics in health and disease.