| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MCJ; HSD18; DNAJD1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DNAJC15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与DNAJC15抗体相关的文献示例(信息基于公开研究整理，非真实文献)：
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1. **文献名称**：*DNAJC15 regulates mitochondrial apoptosis and cancer cell survival*
**作者**：Smith A et al.
**摘要**：本研究利用DNAJC15特异性抗体，发现该蛋白通过调控Bax/Bak依赖性线粒体膜通透性促进细胞凋亡，其表达水平与乳腺癌耐药性相关。
2. **文献名称**：*DNAJC15 interacts with PINK1/Parkin pathway in mitophagy*
**作者**：Chen L et al.
**摘要**：通过免疫共沉淀(使用DNAJC15抗体)和功能实验，证明DNAJC15在帕金森病模型中参与线粒体自噬，与PINK1蛋白协同维持线粒体稳态。
3. **文献名称**：*DNAJC15 deficiency exacerbates metabolic syndrome in mice*
**作者**：Wang Y et al.
**摘要**：利用DNAJC15敲除小鼠及Western blot(抗体验证)，表明DNAJC15缺失导致线粒体功能障碍，促进肥胖和胰岛素抵抗，提示其代谢保护作用。
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注：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索确认。推荐使用关键词 **"DNAJC15 antibody" + "mitochondria"/"apoptosis"/"cancer"** 筛选近期研究。
The DNAJC15 antibody is a research tool designed to detect and study DNAJC15. a member of the DnaJ/Hsp40 protein family. DNAJC15. also known as Tid1 or mitochondrial DnaJ protein, functions as a co-chaperone for Hsp70 proteins, aiding in protein folding, quality control, and mitochondrial homeostasis. It is localized primarily in mitochondria and plays roles in apoptosis, cellular stress responses, and energy metabolism. Dysregulation of DNAJC15 has been linked to cancer, neurodegenerative diseases, and metabolic disorders, making it a target for mechanistic and therapeutic studies.
Antibodies against DNAJC15 are generated using immunogenic peptide regions of the protein, often in hosts like rabbits or mice. These antibodies enable detection of endogenous DNAJC15 in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Validation typically includes knockout/knockdown controls to confirm specificity. Commercial antibodies vary in clonality (monoclonal/polyclonal), epitope targets, and applications, requiring optimization for experimental conditions like fixation or sample preparation.
Research utilizing DNAJC15 antibodies has elucidated its interaction with mitochondrial complexes, involvement in stress-induced apoptosis via the Bcl-2 pathway, and potential as a biomarker in diseases like prostate cancer. However, discrepancies in reported molecular weights (∼15-25 kDa) across studies highlight the importance of antibody validation and consideration of post-translational modifications.
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