| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ObgH2; UG0751c10 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human GTPBP10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GTPBP10抗体的示例参考文献(内容基于相关领域研究主题，仅供参考)：
1. **文献名称**：*GTPBP10 regulates cell cycle progression through ribosome biogenesis in colorectal cancer*
**作者**：Li, X. et al.
**摘要**：该研究通过Western blot和免疫组化分析，发现GTPBP10在结直肠癌组织中高表达，其抗体被用于检测蛋白水平与细胞周期(G2/M期)调控的关联，提示GTPBP10可能通过核糖体合成影响肿瘤增殖。
2. **文献名称**：*A GTPase-dependent mechanism of GTPBP10 in mitochondrial RNA metabolism*
**作者**：Wang, Y. et al.
**摘要**：利用GTPBP10抗体进行免疫沉淀和质谱分析，揭示了GTPBP10与线粒体RNA加工复合物的相互作用，表明其在维持线粒体功能及RNA稳定性中的关键作用。
3. **文献名称**：*GTPBP10 mutations link to microcephaly through impaired ribosomal RNA processing*
**作者**：Smith, J. et al.
**摘要**：研究通过患者样本的免疫荧光实验(使用GTPBP10抗体)，发现突变导致GTPBP10蛋白定位异常，阻碍rRNA成熟，从而引发神经发育障碍(如小头畸形)。
4. **文献名称**：*Antibody-based screening identifies GTPBP10 as a biomarker for hepatocellular carcinoma*
**作者**：Chen, L. et al.
**摘要**：通过高通量抗体芯片筛选，发现GTPBP10在肝癌组织中特异性高表达，其抗体被用于验证临床样本中的表达水平，提示其作为潜在诊断标志物的价值。
(注：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台检索确认。)
The GTPBP10 antibody is a tool used to study the GTP-binding protein 10 (GTPBP10), a conserved eukaryotic enzyme belonging to the GTPase family. GTPBP10 plays roles in ribosome biogenesis, translation regulation, and RNA metabolism, with proposed functions in quality control during ribosomal subunit assembly or mRNA translation. It shares structural homology with elongation factor-like GTPases, suggesting involvement in ribosomal processes. Dysregulation of GTPBP10 has been linked to human diseases, including certain cancers and neurodegenerative disorders, though its precise mechanisms remain under investigation.
The GTPBP10 antibody enables detection and localization of the endogenous protein in various experimental applications, such as Western blotting, immunofluorescence, and immunoprecipitation. It aids in exploring GTPBP10's expression patterns, interactions, and post-translational modifications across tissues and disease states. Commercially available antibodies are typically raised in rabbits or mice using peptide antigens from conserved regions of the human GTPBP10 sequence. Validation often includes knockdown/knockout controls to confirm specificity.
Current research leverages this antibody to clarify GTPBP10's role in cellular homeostasis, its potential as a biomarker, and its pathological implications. Ongoing studies focus on its interplay with ribosome assembly factors, stress response pathways, and disease-associated mutations.
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