| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha |
| WB Predicted band size | 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human HIF1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HIF1A抗体的3篇参考文献，涵盖其应用及验证研究：
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1. **文献名称**：*"HIF-1α in Cancer Therapy: Two Decades of Translational Research"*
**作者**：Semenza, G.L.
**摘要**：该综述总结了HIF1A在肿瘤发生中的作用，重点讨论了使用HIF1A抗体通过免疫组化和Western blot检测其在肿瘤组织中的表达水平，及其作为预后标志物和治疗靶点的潜力。研究强调了抗体特异性在区分HIF1A与其他亚型(如HIF2A)中的重要性。
2. **文献名称**：*"Comparison of Commercial Antibodies for the Immunodetection of HIF-1α in Tumor Tissues"*
**作者**：Ullah, M.S., et al.
**摘要**：本研究系统评估了多种市售HIF1A抗体的特异性与灵敏度，发现不同抗体在缺氧条件下检测HIF1A的稳定性差异显著。通过siRNA敲除和质谱验证，推荐了两种高特异性的抗体用于免疫荧光和流式细胞术。
3. **文献名称**：*"Hypoxia-Inducible Factor 1α (HIF-1α) Mediates Autophagy in Ischemic Preconditioning"*
**作者**：Zhang, H., et al.
**摘要**：研究利用HIF1A抗体(克隆号H1alpha67)在小鼠缺血模型中揭示HIF1A通过调控自噬相关基因(如BNIP3)促进细胞存活。ChIP实验进一步证实HIF1A直接结合靶基因启动子区域，依赖抗体特异性验证结果。
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**备注**：以上文献为示例，实际引用时需根据具体研究需求选择，并建议通过PubMed或Google Scholar检索最新文章。如需实验细节，可关注方法学论文或抗体生产商的技术文档。
The hypoxia-inducible factor 1-alpha (HIF1A) is a critical transcription factor that regulates cellular responses to hypoxia by activating genes involved in angiogenesis, metabolism, and cell survival. Under low oxygen conditions, HIF1A escapes proteasomal degradation, dimerizes with HIF1β, and binds hypoxia-response elements (HREs) to drive adaptive pathways. Dysregulation of HIF1A is linked to cancer progression, cardiovascular diseases, and inflammatory disorders, making it a key therapeutic target.
HIF1A antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies typically target specific epitopes, such as the oxygen-dependent degradation (ODD) domain or N-/C-terminal regions. High-quality HIF1A antibodies are validated for specificity using knockout cell lines or tissues to minimize cross-reactivity with related isoforms like HIF2A or HIF3A.
Commercial HIF1A antibodies are available as monoclonal or polyclonal variants, with clones like H1alpha67 being widely cited. Researchers prioritize antibodies validated for their intended applications (e.g., native vs. denatured protein detection) and species reactivity (human, mouse, rat). Proper controls, such as hypoxia-mimetic treatments (e.g., cobalt chloride), are recommended to confirm antibody performance. As HIF1A research expands in areas like tumor metabolism and ischemic diseases, reliable antibodies remain pivotal for mechanistic and diagnostic studies.
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