| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NELFE |
| Uniprot No | P18615 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMLVIPPGLSEEEEALQKKFNKLKKKKKALL ALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAE TKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRK SLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSA SPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRER DRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAF SPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIA RKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NELFE重组蛋白的参考文献示例(注：内容为虚构示例，仅供格式参考)：
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1. **文献名称**：*Structural characterization of recombinant NELFE protein and its role in transcriptional regulation*
**作者**：Smith J, Lee K, et al.
**摘要**：本研究通过大肠杆菌表达系统纯化了重组NELFE蛋白，并利用X射线晶体学解析其三维结构。实验表明，NELFE与RNA聚合酶II结合后可能通过构象变化抑制转录延伸活性。
2. **文献名称**：*Functional analysis of NELFE in mRNA processing using recombinant protein variants*
**作者**：Chen L, Wang H, et al.
**摘要**：通过构建NELFE重组蛋白的突变体，研究者发现其C端结构域对结合RNA及调控转录终止至关重要，并揭示了其在mRNA加工异常相关疾病中的潜在作用。
3. **文献名称**：*NELFE knockdown and recombinant rescue in cellular models of inflammation*
**作者**：Rodriguez S, Patel R, et al.
**摘要**：利用重组NELFE蛋白进行功能回复实验，证实其通过NF-κB信号通路调控炎症因子表达，为免疫疾病的治疗靶点提供了实验依据。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词“NELFE recombinant protein”或“NELF-E protein structure/function”。
**Background of NELFE Recombinant Protein**
NELFE (Negative Elongation Factor E), also known as RDBP, is a critical component of the NELF (Negative Elongation Factor) complex, which regulates RNA polymerase II (Pol II)-mediated transcriptional elongation. The NELF complex, composed of NELFA, NELFB, NELFE, and either NELFCD or COBRA1. functions in pausing Pol II during early transcription elongation, a key checkpoint for gene expression control. NELFE specifically interacts with nascent RNA transcripts and stabilizes the paused Pol II complex, enabling precise regulation of gene activation in response to cellular signals.
Recombinant NELFE protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional NELFE for *in vitro* and *in vivo* studies. This recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection. Its production enables researchers to dissect NELFE's structural and mechanistic roles in transcription regulation, including its RNA-binding activity, interactions with other NELF subunits, and involvement in developmental or disease-related pathways.
Studies leveraging recombinant NELFE have highlighted its importance in diverse biological processes, such as immune response, cancer progression, and neuronal development. Dysregulation of NELFE has been linked to malignancies and neurological disorders, underscoring its potential as a therapeutic target. Additionally, recombinant NELFE is vital for structural studies (e.g., X-ray crystallography, cryo-EM) and high-throughput screening for drug discovery. By providing a scalable and consistent protein source, recombinant NELFE accelerates research into transcriptional pausing mechanisms and their implications in health and disease.
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