| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MRPL12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL12抗体的参考文献示例(注：以下内容为示例性虚构，仅供参考格式)：
1. **文献名称**: "MRPL12 regulates mitochondrial translation and cell cycle progression via mTOR signaling"
**作者**: Zhang Y, et al.
**摘要**: 本研究利用MRPL12特异性抗体，通过Western blot和免疫荧光技术，发现MRPL12敲低会抑制线粒体核糖体组装，导致氧化磷酸化缺陷，并通过mTOR通路阻滞细胞周期，提示其在肿瘤代谢中的潜在作用。
2. **文献名称**: "Downregulation of MRPL12 in Alzheimer’s disease patient brains correlates with mitochondrial dysfunction"
**作者**: Smith J, et al.
**摘要**: 通过免疫组化分析阿尔茨海默病患者脑组织，发现MRPL12蛋白水平显著降低，且与线粒体复合体活性下降相关，表明其异常表达可能参与神经退行性病变。
3. **文献名称**: "Structural insights into the human mitochondrial ribosome: Role of MRPL12 in subunit assembly"
**作者**: Gupta R, et al.
**摘要**: 使用冷冻电镜和MRPL12抗体进行免疫共沉淀，解析了线粒体核糖体大亚基的结构，揭示了MRPL12在稳定rRNA构象中的关键作用。
4. **文献名称**: "MRPL12 as a prognostic biomarker in colorectal cancer: Evidence from immunohistochemical analysis"
**作者**: Lee H, et al.
**摘要**: 基于组织芯片的免疫组化研究显示，MRPL12在结直肠癌中高表达，且与患者总生存期缩短相关，提示其作为预后标志物的潜力。
**提示**：实际文献需通过PubMed或Google Scholar以关键词“MRPL12 antibody”检索获取。
The mitochondrial ribosomal protein L12 (MRPL12) is a key component of the large subunit of the mitochondrial ribosome (mitoribosome), which is essential for protein synthesis within mitochondria. Encoded by nuclear DNA, MRPL12 is imported into mitochondria and plays a critical role in assembling and stabilizing the mitoribosome structure. Beyond its ribosomal function, MRPL12 interacts with mitochondrial RNA polymerase (POLRMT) to regulate mitochondrial transcription, linking translation and transcription processes in mitochondrial gene expression.
MRPL12 antibodies are widely used tools to investigate mitochondrial dysfunction, energy metabolism, and diseases associated with mitoribosome anomalies, such as cancer, neurodegenerative disorders, and mitochondrial encephalopathies. These antibodies enable the detection of MRPL12 expression levels, subcellular localization (via immunofluorescence or immunohistochemistry), and protein-protein interactions (via co-immunoprecipitation or Western blot). Studies using MRPL12 antibodies have revealed its role in maintaining mitochondrial DNA integrity, oxidative phosphorylation efficiency, and cellular stress responses.
Dysregulation of MRPL12 has been implicated in tumor progression, metabolic syndromes, and aging, making its antibody a valuable reagent for both basic research and clinical diagnostics. Specificity validation (e.g., knockout controls) is critical due to potential cross-reactivity with cytoplasmic ribosomal proteins. Overall, MRPL12 antibodies contribute to understanding mitochondrial biology and its intersection with human health.
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