| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NHLH1 |
| Uniprot No | Q02575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-133aa |
| 氨基酸序列 | MMLNSDTMEL DLPPTHSETE SGFSDCGGGA GPDGAGPGGP GGGQARGPEP GEPGRKDLQH LSREERRRRR RATAKYRTAH ATRERIRVEA FNLAFAELRK LLPTLPPDKK LSKIEILRLA ICYISYLNHV LDV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NHLH1重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，实际引用需核实原文)：
1. **"NHLH1 regulates neurogenic differentiation in murine embryonic stem cells"**
*作者：Goodrich LV, et al. (1996)*
摘要：研究利用重组NHLH1蛋白，证实其通过结合特定DNA序列激活神经分化相关基因，促进小鼠胚胎干细胞向神经元定向分化。
2. **"Functional interaction between NHLH1 and MyoD during myogenesis"**
*作者：Salma J, et al. (2009)*
摘要：通过体外重组蛋白结合实验，发现NHLH1与MyoD形成复合物，协同调控肌肉特异性基因表达，揭示其在成肌细胞分化中的关键作用。
3. **"Post-translational modification of NHLH1 modulates its transcriptional activity"**
*作者：Kurakawa E, et al. (2002)*
摘要：研究重组NHLH1蛋白的磷酸化修饰，证明其通过MAPK通路调控DNA结合能力，影响下游靶基因的转录激活功能。
如需具体文献，建议通过PubMed或Google Scholar以“NHLH1 recombinant protein”为关键词检索最新研究。
**Background of NHLH1 Recombinant Protein**
NHLH1 (Nescient Helix-Loop-Helix 1), also known as HEN1. is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins play critical roles in regulating gene expression during development, particularly in neurogenesis, myogenesis, and cell differentiation. NHLH1 is encoded by the *NHLH1* gene located on human chromosome 12p13.1 and is highly conserved across vertebrates. It binds to E-box DNA motifs (CANNTG) to activate or repress target genes, often interacting with other regulatory proteins to modulate cellular processes such as proliferation, apoptosis, and metabolic homeostasis.
Recombinant NHLH1 protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. This allows studies on its structure, DNA-binding activity, and interactions with co-factors like MYC/MAX complexes or epigenetic modifiers. NHLH1 has been implicated in obesity-related pathways through its regulation of *POMC* (pro-opiomelanocortin) neurons in the hypothalamus, linking it to energy balance and metabolic disorders. Additionally, aberrant NHLH1 expression is observed in certain cancers, suggesting roles in tumor suppression or oncogenesis depending on cellular context.
Research applications of recombinant NHLH1 include investigating its role in stem cell differentiation, neuronal development, and disease models. Its recombinant form enables precise analysis of post-translational modifications (e.g., phosphorylation) that regulate its stability and activity. Tagged versions (e.g., His, GST) simplify purification and detection in assays like EMSA, ChIP, or Western blot. Understanding NHLH1’s molecular mechanisms may advance therapeutic strategies for metabolic syndromes, neurodegenerative diseases, and cancer.
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