| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NHLH2 |
| Uniprot No | Q02577 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-135aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMMLSPDQ AADSDHPSSA HSDPESLGGT DTKVLGSVSD LEPVEEAEGD GKGGSRAALY PHPQQLSREE KRRRRRATAK YRSAHATRER IRVEAFNLAF AELRKLLPTL PPDKKLSKIE ILRLAICYIS YLNHVLDV |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NHLH2重组蛋白的3篇模拟参考文献(基于领域知识推测，非真实文献)：
1. **文献名称**：*"Recombinant NHLH2 Protein Binds E-box Motifs and Regulates Neural Gene Expression in Vitro"*
**作者**：Goodman et al.
**摘要**：研究通过大肠杆菌系统表达重组NHLH2蛋白，验证其与靶基因启动子区E-box元件的结合能力，并发现其可激活神经分化相关基因的转录。
2. **文献名称**：*"Structural Characterization of NHLH2 Recombinant Protein and Its Role in Adipocyte Differentiation"*
**作者**：Wang et al.
**摘要**：利用X射线晶体学解析重组NHLH2蛋白的螺旋-环-螺旋结构域，实验表明其通过调控PPARγ等因子影响脂肪细胞分化进程。
3. **文献名称**：*"NHLH2 Recombinant Protein Interacts with MYCN and Modulates Neuroblastoma Cell Proliferation"*
**作者**：Lee & Martinez
**摘要**：通过重组NHLH2蛋白的体外互作实验，揭示其与致癌蛋白MYCN的物理结合，并抑制神经母细胞瘤细胞的增殖活性。
(注：以上为基于NHLH2已知功能的模拟文献，实际文献需通过PubMed/Google Scholar检索确认。)
**Background of NHLH2 Recombinant Protein**
NHLH2 (nescient helix-loop-helix 2) is a member of the basic helix-loop-helix (bHLH) family of transcription factors, which play critical roles in cell differentiation, development, and tissue-specific gene regulation. NHLH2 is encoded by the *NHLH2* gene, located on human chromosome 1p22.3. and is highly conserved across vertebrates. Structurally, it contains a bHLH domain that facilitates DNA binding and dimerization with other bHLH proteins to regulate target gene expression. NHLH2 is predominantly expressed in the nervous system, testes, and adrenal glands, and has been implicated in neurogenesis, energy homeostasis, and reproductive functions.
Recombinant NHLH2 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of native NHLH2. enabling its use in *in vitro* studies to dissect molecular mechanisms, such as DNA-binding activity, protein-protein interactions, or transcriptional regulation of downstream genes. For example, NHLH2 is known to regulate *MC4R* (melanocortin-4 receptor), a key player in appetite control, linking it to obesity-related research.
Research on NHLH2 has expanded due to its potential roles in cancer, neurodevelopmental disorders, and metabolic syndromes. Its recombinant form is essential for producing antibodies, validating binding partners via pull-down assays, and screening small molecules that modulate its activity. Challenges remain in understanding tissue-specific regulatory networks and its interplay with epigenetic factors. Advances in recombinant protein technology continue to support deeper exploration of NHLH2’s physiological and pathological significance.
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