| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RP-C; PP2Ac; PP2CA; PP2Calpha |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PPP2CA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP2CA抗体的3篇参考文献信息，按文献名称、作者和摘要内容概括整理：
1. **文献名称**：*"PP2A holoenzyme assembly and function in cancer progression"*
**作者**：Sangodkar J et al.
**摘要**：该研究探讨蛋白磷酸酶PP2A复合体(包括PPP2CA亚基)在癌症中的作用，通过特异性抗体验证PPP2CA在肿瘤组织中的表达变化，揭示其调控细胞增殖和凋亡的机制。
2. **文献名称**：*"Structural insights into PP2A phosphatase assembly and regulatory post-translational modifications"*
**作者**：Xu Y et al.
**摘要**：利用PPP2CA抗体进行免疫共沉淀和质谱分析，解析PP2A全酶复合体的三维结构，阐明其催化亚基与调节亚基的相互作用及翻译后修饰对酶活性的影响。
3. **文献名称**：*"Dysregulation of PP2A in Alzheimer's disease: Role of amyloid-β and tau phosphorylation"*
**作者**：Martin L et al.
**摘要**：通过Western blot和免疫组化实验，使用PPP2CA抗体检测阿尔茨海默病患者脑组织中PP2A活性下降，证明其与tau蛋白异常磷酸化及神经元退化的关联。
(注：以上文献信息为示例，具体引用时需核对真实文献来源及发表年份。)
The PPP2CA antibody is a crucial tool for studying the protein phosphatase 2 catalytic subunit alpha (PPP2CA), a key component of the serine/threonine phosphatase PP2A holoenzyme. PP2A regulates diverse cellular processes, including cell cycle progression, apoptosis, DNA repair, and metabolic signaling, by dephosphorylating target proteins. PPP2CA, encoded by the PPP2CA gene, forms the enzymatic core of PP2A and interacts with regulatory B subunits and scaffolding proteins to determine substrate specificity. Dysregulation of PPP2CA is implicated in cancer, neurodegenerative disorders, and cardiovascular diseases, underscoring its role as a tumor suppressor and signaling modulator.
Antibodies targeting PPP2CA enable the detection, localization, and functional analysis of this subunit in techniques like Western blotting, immunoprecipitation, and immunohistochemistry. They are critical for investigating PP2A’s involvement in pathways such as Ras/MAPK and PI3K/Akt/mTOR. Many PPP2CA antibodies are developed in hosts like rabbits or mice, with monoclonal and polyclonal variants available. Specificity is validated using knockout cell lines or siRNA knockdown to confirm target recognition. Researchers must consider epitope regions, post-translational modifications (e.g., methylation at Leu309), and cross-reactivity with homologous subunits (e.g., PPP2CB) when selecting antibodies. These tools are indispensable for elucidating PP2A’s complex regulatory mechanisms and its therapeutic potential in disease.
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