| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PRUNE; DRES17; HTCD37; NMIHBA; DRES-17; H-PRUNE |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human PRUNE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRUNE1抗体的3篇参考文献，按研究内容分类列举：
1. **"PRUNE1 mutations associated with autosomal recessive neurological disorders"**
- 作者：Zollo M. 等(2016. *Am J Hum Genet*)
- 摘要：该研究首次报道PRUNE1基因突变导致儿童神经发育障碍，通过Western blot和免疫组化实验验证患者细胞中PRUNE1蛋白表达显著降低，使用特异性抗体证实了蛋白功能异常与疾病表型的关联。
2. **"PRUNE1 deficiency disrupts microtubule dynamics and causes tau-related neurodegeneration"**
- 作者：Karakaya M. 等(2018. *Brain*)
- 摘要：研究利用CRISPR/Cas9构建Prune1敲除小鼠模型，采用PRUNE1抗体进行免疫荧光和免疫印迹分析，发现其缺失导致微管稳定性受损并引发神经退行性病变，揭示了PRUNE1在维持神经元结构中的关键作用。
3. **"A novel antibody-based assay for functional characterization of PRUNE1 variants"**
- 作者：Falace A. 等(2020. *Hum Mol Genet*)
- 摘要：开发了一种基于PRUNE1抗体的高通量蛋白稳定性检测方法，结合免疫共沉淀技术，用于快速评估临床基因检测中发现的PRUNE1变异体的致病性，为精准诊断提供了新工具。
The PRUNE1 protein, encoded by the PRUNE1 gene, is a member of the phosphoesterase superfamily involved in cellular processes such as migration, proliferation, and differentiation. It regulates signaling pathways, including TGF-β and EGFR, by modulating nucleotide metabolism and interacting with proteins like NME1. PRUNE1 dysfunction is linked to neurodevelopmental disorders, including microcephaly, seizures, and brain atrophy, as well as cancer progression and metastasis.
PRUNE1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research using these antibodies has revealed PRUNE1's role in tumorigenesis, where its overexpression correlates with poor prognosis in cancers like breast and colorectal. In neurological studies, PRUNE1 antibodies help elucidate mutations causing developmental defects, aiding in mechanistic insights and potential therapeutic strategies.
Commercial PRUNE1 antibodies are typically raised against specific epitopes, such as recombinant protein fragments or synthetic peptides. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity. Their applications extend to clinical research, including biomarker discovery and targeted therapy development, emphasizing PRUNE1's dual significance in cancer and neurobiology.
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