| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | FNRA; CD49e; VLA5A; ITGA5 |
| Entrez GeneID | 3678 |
| clone | 10F6 |
| WB Predicted band size | 114kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ITGA5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PSMC1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Proteasome function is regulated by reversible phosphorylation*
**作者**：Satoh K, Sasajima H, Nyoumura KI
**摘要**：该研究探讨蛋白酶体功能与磷酸化的关系，使用PSMC1抗体进行免疫共沉淀实验，发现PSMC1亚基的磷酸化状态影响蛋白酶体活性，提示其在细胞周期调控中的作用。
2. **文献名称**：*Targeting PSMC1 inhibits multiple myeloma growth by disrupting proteostasis*
**作者**：Hideshima T, et al.
**摘要**：研究通过PSMC1抗体阻断蛋白酶体功能，证明在多发性骨髓瘤中抑制PSMC1可诱导未折叠蛋白反应，导致癌细胞凋亡，支持其作为治疗靶点的潜力。
3. **文献名称**：*Characterization of 26S proteasome subunits in Arabidopsis thaliana*
**作者**：Yao Y, et al.
**摘要**：利用PSMC1抗体对拟南芥26S蛋白酶体进行亚细胞定位分析，揭示了其在植物胁迫响应中的表达变化及蛋白质降解机制。
(注：以上文献信息为模拟示例，实际引用请以真实论文为准。)
The PSMC1 (Proteasome 26S Subunit, ATPase 1) antibody targets a critical component of the 26S proteasome, a large multiprotein complex responsible for regulated protein degradation in eukaryotic cells. PSMC1. also known as Rpt2. is part of the 19S regulatory particle that binds to the 20S core to form the 26S proteasome. It functions as an ATPase, providing energy for substrate unfolding and translocation into the proteolytic core. PSMC1 plays a key role in maintaining protein homeostasis, regulating processes like cell cycle progression, DNA repair, and stress response by degrading ubiquitinated proteins.
Antibodies against PSMC1 are widely used to study proteasome composition, dynamics, and dysfunction in diseases. They enable detection of PSMC1 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Research applications include investigating proteasome activity in cancer (where proteasomes are often hyperactive), neurodegenerative disorders (linked to protein aggregation), and immune responses. Altered PSMC1 expression has been associated with drug resistance in chemotherapy and the efficacy of proteasome inhibitors like bortezomib. These antibodies also help explore evolutionary conservation of the ubiquitin-proteasome system across species. Proper validation ensures specificity for distinguishing PSMC1 from other AAA-ATPase family members in the 19S complex.
×
