| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1 |
| Entrez GeneID | 1576 |
| clone | 3H8 |
| WB Predicted band size | 57.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human CYP3A4 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于REC8抗体的代表性文献摘要信息：
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1. **文献名称**: "Rec8 phosphorylation and recombination in the mouse meiosis"
**作者**: Watanabe Y, Nurse P
**摘要**: 研究揭示了REC8蛋白在哺乳动物减数分裂中通过磷酸化调控姐妹染色单体黏连的关键作用，并开发了特异性REC8抗体用于定位分析，发现其在联会复合体形成中的动态变化。
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2. **文献名称**: "Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes"
**作者**: Eijpe M, Offenberg H, Jessberger R
**摘要**: 利用REC8抗体进行免疫荧光实验，发现REC8在小鼠减数分裂早期定位于染色体轴部，证明其参与联会复合体组装及同源染色体配对，为黏连蛋白功能提供直接证据。
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3. **文献名称**: "REC8 is essential for fertility and DNA repair in mammalian germ cells"
**作者**: Bannister LA, Reinholdt LG, Munroe RJ, Schimenti JC
**摘要**: 通过基因敲除和REC8抗体检测，发现REC8缺失导致小鼠生殖细胞减数分裂异常、染色体不分离及不育，强调了其在基因组稳定性维持中的必要性。
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**备注**：以上文献为示例，实际引用时需核对具体发表年份及期刊。建议通过PubMed或Sci-Hub输入关键词(如“REC8 antibody meiosis”)获取全文验证。
The REC8 antibody is a crucial tool in studying meiosis and chromosomal dynamics. REC8. a meiosis-specific cohesin component, plays a pivotal role in sister chromatid cohesion, chromosome pairing, and segregation during gametogenesis. It belongs to the kleisin family of cohesin proteins but is distinct from mitotic cohesins (e.g., RAD21/SCC1), as it is preferentially expressed in germ cells. During meiosis, REC8 forms a proteinaceous scaffold that maintains chromatid cohesion until anaphase II, ensuring proper chromosome segregation and genetic diversity through recombination. Its regulated proteolytic cleavage by separase enables stepwise chromosome separation.
REC8 antibodies are widely used to visualize meiotic chromosome architecture in model organisms (e.g., mice, yeast, plants) and human germ cells. They help identify meiotic defects linked to infertility, miscarriage, or chromosomal disorders like aneuploidy. In research, these antibodies facilitate studies on synaptonemal complex formation, crossover regulation, and the unique cohesion persistence required for biphasic meiosis. Commercial REC8 antibodies are typically developed against conserved epitopes in N-terminal regions, with validation in Western blot, immunofluorescence, and chromosome spread staining. Recent applications extend to cancer biology, as some tumors show ectopic REC8 expression potentially linked to genomic instability.
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