| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RBQ3; SWD1 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RBBP5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及RBBP5抗体的文献概述(基于近年研究领域推测，部分信息可能需核实)：
1. **文献名称**：RBBP5 modulates histone H3K4 methylation and gene expression in leukemia maintenance
**作者**：Wang et al. (2018)
**摘要**：研究利用RBBP5抗体通过ChIP-seq分析其在白血病细胞中与H3K4甲基化复合物的结合位点，揭示RBBP5通过调控关键致癌基因的表观修饰维持肿瘤恶性表型。
2. **文献名称**：Structural basis for RBBP5 interaction with MLL1 histone methyltransferase complex
**作者**：Chen & Zhang (2020)
**摘要**：通过免疫共沉淀(Co-IP)结合RBBP5抗体，解析了RBBP5与MLL复合物的结构互作机制，阐明其在组蛋白甲基转移酶活性调控中的关键作用。
3. **文献名称**：RBBP5 silencing impairs DNA damage response via defective BRCA1 recruitment
**作者**：Li et al. (2021)
**摘要**：研究使用RBBP5抗体进行Western blot和免疫荧光实验，发现其通过介导BRCA1的染色质定位参与DNA损伤修复，缺失导致基因组不稳定。
*注：以上为模拟文献，实际文献需通过PubMed/Google Scholar检索关键词“RBBP5 antibody”或“RBBP5 function”获取。建议优先选择近5年高分期刊(如Nature, Cell, PNAS)中明确描述抗体应用(如WB/IP/IHC)的研究。*
The RBBP5 (Retinoblastoma-Binding Protein 5) antibody is a critical tool for studying epigenetic regulation and transcriptional control. RBBP5 is a conserved nuclear protein that plays a scaffolding role in multi-subunit histone methyltransferase complexes, including the SET1/MLL family complexes responsible for catalyzing histone H3 lysine 4 (H3K4) methylation—a hallmark of active gene promoters. By interacting with proteins like MLL1/KMT2A and ASH2L, RBBP5 stabilizes these complexes and enhances their enzymatic activity, influencing processes such as embryonic development, hematopoiesis, and cellular differentiation. Dysregulation of RBBP5 is linked to cancers, developmental disorders, and other diseases.
Antibodies targeting RBBP5 are widely used in research to investigate its expression, localization, and molecular interactions. They enable techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP), helping dissect its role in chromatin remodeling and gene regulation. Validated RBBP5 antibodies typically show specificity in detecting the ~60 kDa protein across human, mouse, and rat samples, often confirmed via knockout (KO) controls. These reagents are essential for exploring RBBP5's involvement in disease mechanisms or its crosstalk with signaling pathways, supporting advances in epigenetics and therapeutic discovery.
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