| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOJ1; C12orf2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RASSF8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RASSF8抗体的参考文献示例(注：部分文献信息为示例性概括，建议通过学术数据库进一步验证)：
1. **文献名称**: "RASSF8 regulates cadherin-based adherens junctions and maintains epithelial polarity"
**作者**: Richter AM, et al.
**摘要**: 该研究利用RASSF8抗体进行免疫荧光染色，发现RASSF8通过调控E-cadherin的稳定性参与上皮细胞极性的维持，并揭示其在抑制肿瘤细胞侵袭中的作用。
2. **文献名称**: "Epigenetic inactivation of RASSF8 in lung cancer and its functional implications"
**作者**: Dallol A, et al.
**摘要**: 通过Western blot和免疫组化分析RASSF8抗体标记的蛋白表达，发现RASSF8在肺癌中因启动子甲基化而表达下调，其缺失促进细胞增殖和迁移。
3. **文献名称**: "RASSF8 interacts with the tight junction protein ZO-1 and modulates barrier function"
**作者**: Vlahov N, et al.
**摘要**: 研究采用共聚焦显微镜和Co-IP技术，结合RASSF8抗体证实其与ZO-1的相互作用，表明RASSF8通过维持紧密连接完整性影响肠道屏障功能。
4. **文献名称**: "RASSF8 suppresses EMT by stabilizing cell-cell junctions via the Hippo pathway"
**作者**: Liu Y, et al.
**摘要**: 利用RASSF8抗体进行组织芯片分析，发现RASSF8通过激活Hippo信号通路抑制上皮-间质转化(EMT)，在乳腺癌中具有抑癌作用。
**建议**：以上内容为基于领域知识的模拟文献，具体文献请通过PubMed、Google Scholar等平台以“RASSF8 antibody”或“RASSF8 function”为关键词检索获取。
The RASSF8 antibody is a crucial tool for studying the Ras association domain family member 8 (RASSF8), a protein implicated in cellular adhesion, signaling, and tumor suppression. RASSF8 belongs to the RASSF family, which interacts with Ras GTPases to regulate pathways controlling cell proliferation, apoptosis, and cytoskeletal organization. Structurally, RASSF8 contains a conserved Ras-association (RA) domain and a coiled-coil domain, enabling interactions with membrane-associated proteins and cytoskeletal components. It localizes to cell-cell junctions and plays roles in maintaining epithelial integrity, cell migration, and polarity. Dysregulation of RASSF8 has been linked to cancers, including lung and colorectal malignancies, where its downregulation may promote metastasis.
The RASSF8 antibody, typically generated in rabbits or mice using immunogenic peptide sequences, allows detection and quantification of endogenous RASSF8 via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Its specificity is validated using knockout controls or siRNA-mediated silencing to confirm minimal cross-reactivity with other RASSF members. Researchers employ this antibody to explore RASSF8's involvement in Hippo and MAPK signaling pathways, its tumor-suppressive functions, and its interplay with adherens junction proteins like E-cadherin. By enabling spatial and temporal analysis of RASSF8 expression, the antibody aids in elucidating its role in developmental biology, tissue homeostasis, and cancer progression, with potential applications in diagnostic or therapeutic biomarker discovery.
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