| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human RASA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RASA1抗体的参考文献示例(注：以下文献信息为示例性内容，非真实存在的论文)：
1. **文献名称**: "RASA1-Specific Monoclonal Antibody Development and Application in Vascular Malformation Studies"
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对RASA1蛋白的高特异性单克隆抗体，并通过Western blot和免疫组织化学验证其在人和小鼠组织中的适用性。研究利用该抗体揭示了RASA1在遗传性血管畸形患者组织中的表达缺失，支持其在调节内皮细胞RAS信号通路中的关键作用。
2. **文献名称**: "RASA1 Antibody-Based Detection of Protein Localization in Developmental Angiogenesis"
**作者**: Chen L, et al.
**摘要**: 通过免疫荧光和共聚焦显微镜技术，使用RASA1多克隆抗体分析了小鼠胚胎血管系统的蛋白分布。研究发现RASA1在血管内皮细胞中高表达，且其空间定位与VEGF信号通路活性相关，提示其通过调控RAS-MAPK通路抑制过度血管生成。
3. **文献名称**: "Functional Characterization of RASA1 Antibody in Neurofibromatosis Type 1 Models"
**作者**: Gupta R, et al.
**摘要**: 本研究利用商业化RASA1抗体探究了神经纤维瘤病1型(NF1)模型中RASA1与神经纤维蛋白的相互作用。抗体通过免疫沉淀证实了RASA1在NF1缺失细胞中的异常聚集，为靶向RAS信号的治疗策略提供了实验依据。
4. **文献名称**: "RASA1 Expression Profiling in Cancer: Validation of a Novel Antibody for Clinical Biomarker Studies"
**作者**: Kim H, et al.
**摘要**: 验证了一种新型兔源RASA1抗体在结直肠癌组织芯片中的可靠性，发现RASA1低表达与肿瘤侵袭性和KRAS突变显著相关。该抗体被推荐为潜在预后标志物检测工具。
(注：实际文献需通过PubMed、Google Scholar等平台以“RASA1 antibody”或“RASA1 immunodetection”为关键词检索获取。)
The RASA1 antibody is designed to detect RAS p21 protein activator 1 (RASA1), a critical regulatory protein involved in the RAS/MAPK signaling pathway. RASA1. also known as p120-RasGAP, functions as a GTPase-activating protein (GAP) that negatively regulates RAS activity by accelerating its intrinsic GTPase activity, thereby converting active GTP-bound RAS to its inactive GDP-bound form. This regulation is essential for controlling cellular processes such as proliferation, differentiation, and apoptosis. Mutations or dysregulation of RASA1 are linked to vascular malformations, including capillary malformation-arteriovenous malformation (CM-AVM) syndrome, and have been implicated in certain cancers due to aberrant RAS signaling.
RASA1 antibodies are widely used in research to study protein expression, localization, and function in various tissues and disease models. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These tools help elucidate RASA1's role in vascular development, tumor suppression, and signal transduction mechanisms. Additionally, RASA1 antibodies contribute to diagnostic investigations of genetic disorders and potential therapeutic strategies targeting RAS pathway dysregulation. Their specificity and reliability make them vital for advancing understanding of RASA1-related pathologies and cellular signaling networks.
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