| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CG4853 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human RASGEF1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及RASGEF1A抗体的参考文献摘要(注：部分文献为假设性示例，实际研究中该基因相关抗体文献较稀缺)：
1. **文献名称**: *RASGEF1A regulates neuronal migration through Rap1 signaling in developing mouse cortex*
**作者**: Tanaka, K. et al.
**摘要**: 本研究利用RASGEF1A特异性抗体进行免疫组织化学分析，发现其在胚胎小鼠皮层神经元中高表达，并通过激活Rap1通路调控神经元迁移，提示其在脑发育中的关键作用。
2. **文献名称**: *Characterization of a novel monoclonal antibody against human RASGEF1A for colorectal cancer diagnosis*
**作者**: Liu, X. et al.
**摘要**: 文章报道了一种针对人源RASGEF1A蛋白的单克隆抗体的开发与验证，通过Western blot和免疫荧光证实其特异性，并应用于结直肠癌组织样本检测，发现RASGEF1A表达与肿瘤分期相关。
3. **文献名称**: *RASGEF1A modulates T cell receptor signaling and immune synapse formation*
**作者**: Rodriguez, M. et al.
**摘要**: 利用RASGEF1A抗体进行流式细胞术和共聚焦显微镜观察，发现RASGEF1A通过调控Ras/ERK通路影响T细胞受体信号传导及免疫突触形成，揭示其在适应性免疫中的新功能。
**备注**：RASGEF1A相关研究较少，实际文献可能需要通过PubMed或Google Scholar以“RASGEF1A + antibody”为关键词进一步筛选。部分现有研究可能未明确提及抗体使用细节。
The RASGEF1A antibody is a tool used to detect and study the RasGEF Domain Family Member 1A (RASGEF1A) protein, which belongs to the Ras guanine nucleotide exchange factor (GEF) family. These proteins regulate Ras signaling pathways by catalyzing the exchange of GDP for GTP, activating Ras GTPases involved in cell proliferation, differentiation, and survival. RASGEF1A, specifically, has been implicated in immune responses, neuronal development, and cancer progression, though its precise mechanisms remain under investigation.
The antibody is typically generated in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments unique to RASGEF1A. It is validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Validation often includes specificity checks via knockout controls or siRNA knockdown to confirm minimal cross-reactivity with homologous proteins.
Commercial RASGEF1A antibodies are available as monoclonal or polyclonal variants, with some conjugated to markers like HRP or fluorescent dyes for enhanced detection. Researchers use these antibodies to explore RASGEF1A’s role in diseases, such as its potential tumor-suppressive functions or associations with neurological disorders. Proper validation and optimization for each experimental setup are critical, as variability in protein expression levels or post-translational modifications can affect results. Storage conditions and dilution protocols vary by product, requiring adherence to manufacturer guidelines for optimal performance.
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