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Mouse Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit

  • 中文名: 小鼠清道夫受体富含半胱氨酸1蛋白M130(CD163)酶联免疫试剂盒
货号: IPD-EL004882MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠清道夫受体富含半胱氨酸1蛋白M130(CD163)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CD163含量。CD163是一种清道夫受体,主要在单核-巨噬细胞膜上表达,具有清除血红蛋白-触珠蛋白复合物的功能。它既是巨噬细胞活化的标志物,也具有抗炎、抗氧化作用,与肿瘤、肝衰竭等疾病的发生发展密切相关。试剂盒检测范围为0.93 ng/ml-60ng/ml,灵敏度为0.23 ng/ml。适用于炎症模型研究、免疫调控机制探索、血液相关疾病动物实验等科研场景,能够有效评估巨噬细胞活化状态或病理条件下CD163的动态变化,为心血管疾病、代谢综合征及感染性疾病的基础研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Cd163 ELISA kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA kit; sCD163)] ELISA kit
  • 缩写:
    CD163
  • Uniprot No.:
    Q2VLH6
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.9 ng/ml-60 ng/ml
  • 灵敏度:
    0.23 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    91-103


    1:2

    Average %

    87


    Range %

    80-96


    1:4

    Average %

    87


    Range %

    84-91


    1:8

    Average %

    95


    Range %

    91-99


  • 回收率:

    The recovery of mouse CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    88

    82-95


    EDTA plasma (n=4)

    92

    87-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    60

    2.306

    2.233

    2.270

    2.206


    30

    1.356

    1.275

    1.316

    1.252


    15

    0.742

    0.752

    0.747

    0.683


    7.5

    0.481

    0.491

    0.486

    0.422


    3.75

    0.264

    0.275

    0.270

    0.206


    1.8

    0.207

    0.205

    0.206

    0.142


    0.9

    0.105

    0.100

    0.103

    0.039


    0

    0.063

    0.065

    0.064




  • 货期:
    3-5 working days

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