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产品描述：人Septin-2(SEPT2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SEPT2含量。SEPT2 是一个值得关注的靶点。它属于 Septin 家族，在细胞分裂、细胞骨架形成等多种细胞过程中发挥作用。研究常围绕其在肿瘤发生发展中的机制展开，探讨其与肿瘤细胞增殖、迁移、侵袭等行为的关系，有望为肿瘤治疗提供新方向。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研领域中对SEPT2蛋白表达的定量分析，例如探究其在细胞周期调控、肿瘤微环境变化或神经细胞功能研究中的作用机制，也可用于评估不同实验条件下（如药物处理、基因编辑）SEPT2的表达动态变化，为相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SEPTIN2 ELISA Kit; DIFF6 ELISA Kit; KIAA0158 ELISA Kit; NEDD5 ELISA Kit; SEPT2 ELISA Kit; Septin-2 ELISA Kit; Neural precursor cell expressed developmentally down-regulated protein 5 ELISA Kit; NEDD-5 ELISA Kit
缩写：SEPT2
Uniprot No.：Q15019
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEPT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 84 | |
| Range % | 81-89 | ||
| 1:2 | Average % | 98 | |
| Range % | 93-101 | ||
| 1:4 | Average % | 83 | |
| Range % | 80-86 | ||
| 1:8 | Average % | 99 | |
| Range % | 94-102 |
回收率：
| The recovery of human SEPT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 108 | 104-112 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 16002.2072.2472.2272.080 8001.7861.7981.7921.645 4001.3651.3081.3371.190 2000.8140.8420.8280.681 1000.5920.5680.5800.433 500.3550.3670.3610.214 250.2480.2380.2430.096 00.1460.1480.147 | ||||||
货期：3-5 working days
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