首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠促肾上腺皮质激素(ACTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ACTH含量。ACTH即促肾上腺皮质激素，由腺垂体分泌。其背景在于它能促进肾上腺皮质的组织增生以及皮质激素的生成和分泌。研究机制方面，它作用于肾上腺皮质细胞上的受体，激活相关信号通路，调控皮质激素合成关键酶的表达，进而调节机体应激等生理过程。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品适用于实验动物模型中ACTH动态变化的分析，例如应激反应评估、肾上腺功能研究、内分泌调控机制探索以及药物或环境因素对激素分泌影响的科研实验，为神经内分泌领域的定量研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 85-104 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 82-110 | |||||
回收率：
| The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 84-102 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12003.1343.1263.1302.9886002.5372.5322.5352.3933001.7541.7651.7601.6181501.0531.0501.0520.910750.6510.6470.6490.50737.50.4430.4350.4390.29718.750.2730.2650.2690.12700.1400.1440.142 | ||||||
货期：3-5 working days
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