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产品描述：人ADAMTS样蛋白4(ADAMTSL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTSL4含量。ADAMTSL4是一种具有血小板反应蛋白基序的解聚素和金属蛋白酶4，与系统性硬化症（SSc）和肺动脉高压（PAH）的预测和诊断相关。其研究机制涉及在SSc患者中检测ADAMTSL4抗体水平，以预测PAH的发生可能性。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探索ADAMTSL4在组织发育、疾病机制或分子调控网络中的作用；为研究ADAMTSL4在体液中的表达动态或不同组织中的分布特征提供可靠工具；可应用于细胞实验、疾病模型研究或生物标志物筛选等非临床领域的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAMTSL4 ELISA kit; TSRC1 ELISA kit; PP1396 ELISA kit; UNQ2803/PRO34012ADAMTS-like protein 4 ELISA kit; ADAMTSL-4 ELISA kit; Thrombospondin repeat-containing protein 1 ELISA kit
缩写：ADAMTSL4
Uniprot No.：Q6UY14
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTSL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-104 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of human ADAMTSL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 81-87 | |||||
| EDTA plasma (n=4) | 96 | 93-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.0441.9682.0061.84051.7531.7751.7641.5982.51.4151.4021.4091.2431.251.0041.0841.0440.8780.6250.7120.7000.7060.5400.3120.5230.5050.5140.3480.1560.3010.3120.3070.14100.1670.1640.166 | |||||||
货期：3-5 working days
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