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产品描述：人同种异体移植炎症因子1(AIF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AIF1含量。AIF1即离子钙结合衔接子分子1，是一种在活化巨噬细胞中表达的钙结合蛋白。研究发现它参与免疫反应调节，与炎症、自身免疫性疾病及肿瘤发生发展相关。其机制可能是调节巨噬细胞功能及相关信号通路，为疾病治疗提供潜在靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本产品适用于基础科研中AIF1相关机制研究，如炎症性疾病动物模型验证、免疫细胞功能分析或病理组织样本中炎症因子动态监测，为探索AIF1在免疫调控、组织修复及疾病发生发展中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIF 1 ELISA Kit; AIF-1 ELISA Kit; Aif1 ELISA Kit; AIF1 protein ELISA Kit; AIF1_HUMAN ELISA Kit; Allograft inflammatory factor 1 ELISA Kit; Allograft inflammatory factor 1 splice variant G ELISA Kit; allograft inflammatory factor-1 splice variant Hara-1 ELISA Kit; balloon angioplasty responsive transcription ELISA Kit; BART 1 ELISA Kit; G1 ELISA Kit; G1 putative splice variant of allograft inflamatory factor 1 ELISA Kit; IBA 1 ELISA Kit; IBA1 ELISA Kit; interferon gamma responsive transcript ELISA Kit; Interferon responsive transcript 1 ELISA Kit; interferon responsive transcript factor 1 ELISA Kit; Ionized calcium binding adapter molecule 1 ELISA Kit; Ionized calcium-binding adapter molecule 1 ELISA Kit; ionized calcium-binding adapter molecule ELISA Kit; IRT 1 ELISA Kit; IRT1 ELISA Kit; Microglia response factor ELISA Kit; MRF1 ELISA Kit; Protein g1 ELISA Kit
缩写：AIF1
Uniprot No.：P55008
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AIF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 104 | |||||
| Range % | 101-108 | ||||||
| 1:10 | Average % | 91 | |||||
| Range % | 88-97 | ||||||
| 1:20 | Average % | 96 | |||||
| Range % | 91-104 | ||||||
| 1:40 | Average % | 89 | |||||
| Range % | 86-93 | ||||||
回收率：
| The recovery of human AIF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 99-106 | |||||
| EDTA plasma (n=4) | 96 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected5002.5322.5222.5272.3472502.0882.0272.0581.8781251.3691.4591.4141.23462.50.7840.7790.7820.60231.20.4450.4800.4630.28315.60.2870.2990.2930.1137.80.2130.2300.2220.04200.1750.1850.180 | |||||||
货期：3-5 working days
产品描述：人同种异体移植炎症因子1(AIF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AIF1含量。AIF1即离子钙结合衔接子分子1，是一种在活化巨噬细胞中表达的钙结合蛋白。研究发现它参与免疫反应调节，与炎症、自身免疫性疾病及肿瘤发生发展相关。其机制可能是调节巨噬细胞功能及相关信号通路，为疾病治疗提供潜在靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本产品适用于基础科研中AIF1相关机制研究，如炎症性疾病动物模型验证、免疫细胞功能分析或病理组织样本中炎症因子动态监测，为探索AIF1在免疫调控、组织修复及疾病发生发展中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIF 1 ELISA Kit; AIF-1 ELISA Kit; Aif1 ELISA Kit; AIF1 protein ELISA Kit; AIF1_HUMAN ELISA Kit; Allograft inflammatory factor 1 ELISA Kit; Allograft inflammatory factor 1 splice variant G ELISA Kit; allograft inflammatory factor-1 splice variant Hara-1 ELISA Kit; balloon angioplasty responsive transcription ELISA Kit; BART 1 ELISA Kit; G1 ELISA Kit; G1 putative splice variant of allograft inflamatory factor 1 ELISA Kit; IBA 1 ELISA Kit; IBA1 ELISA Kit; interferon gamma responsive transcript ELISA Kit; Interferon responsive transcript 1 ELISA Kit; interferon responsive transcript factor 1 ELISA Kit; Ionized calcium binding adapter molecule 1 ELISA Kit; Ionized calcium-binding adapter molecule 1 ELISA Kit; ionized calcium-binding adapter molecule ELISA Kit; IRT 1 ELISA Kit; IRT1 ELISA Kit; Microglia response factor ELISA Kit; MRF1 ELISA Kit; Protein g1 ELISA Kit
缩写：AIF1
Uniprot No.：P55008
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AIF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 104 | |||||
| Range % | 101-108 | ||||||
| 1:10 | Average % | 91 | |||||
| Range % | 88-97 | ||||||
| 1:20 | Average % | 96 | |||||
| Range % | 91-104 | ||||||
| 1:40 | Average % | 89 | |||||
| Range % | 86-93 | ||||||
回收率：
| The recovery of human AIF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 99-106 | |||||
| EDTA plasma (n=4) | 96 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected5002.5322.5222.5272.3472502.0882.0272.0581.8781251.3691.4591.4141.23462.50.7840.7790.7820.60231.20.4450.4800.4630.28315.60.2870.2990.2930.1137.80.2130.2300.2220.04200.1750.1850.180 | |||||||
货期：3-5 working days
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