| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MLA1; ME491; LAMP-3; OMA81H; TSPAN30 |
| Entrez GeneID | 967 |
| clone | 1E8D4 |
| WB Predicted band size | 25.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD63 (AA: extra 103-203) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于VAMP4抗体的3篇参考文献示例(注：文献为示例，非真实存在)：
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1. **"A monoclonal antibody targeting VAMP4 reveals its role in Golgi-lysosome trafficking"**
*作者：Tanaka K. et al.*
**摘要**：本研究开发了一种特异性识别VAMP4的单克隆抗体，通过免疫印迹和免疫荧光验证其特异性，揭示了VAMP4在高尔基体-溶酶体膜融合中的关键作用，为研究细胞内运输机制提供新工具。
2. **"VAMP4 antibody-based profiling identifies dysregulation in Alzheimer's disease models"**
*作者：Chen L. et al.*
**摘要**：利用高特异性VAMP4抗体对阿尔茨海默病模型脑组织进行分析，发现VAMP4在淀粉样斑块周围神经元中的异常聚集，提示其可能参与疾病相关的囊泡运输障碍。
3. **"Characterization of a polyclonal anti-VAMP4 antibody for synaptic vesicle studies"**
*作者：Müller S. et al.*
**摘要**：报道一种新型多克隆抗体的制备与验证，该抗体可特异性结合VAMP4的N端结构域，成功应用于突触小泡分离实验及神经元中的共定位分析，支持VAMP4在神经分泌中的功能研究。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“VAMP4 antibody”或“VAMP4 vesicle”，并筛选涉及抗体开发、验证或应用的实验研究。
**Background of VAMP4 Antibody**
VAMP4 (Vesicle-Associated Membrane Protein 4) is a member of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) family, which plays a critical role in intracellular vesicle trafficking and membrane fusion. Specifically, VAMP4 is localized to the trans-Golgi network (TGN) and endosomal compartments, where it regulates secretory pathways, lysosomal sorting, and receptor recycling. Unlike other VAMP isoforms (e.g., VAMP1/2), VAMP4 is enriched in non-neuronal tissues but is also expressed in certain neuronal populations, suggesting tissue-specific functions.
VAMP4 antibodies are essential tools for studying the protein’s expression, localization, and interaction partners. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate VAMP4’s role in cellular processes such as neurotransmitter release, hormone secretion, and autophagy. Dysregulation of VAMP4 has been implicated in neurological disorders (e.g., Alzheimer’s disease), endocrine dysfunction, and cancer, making its study clinically relevant.
These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes within VAMP4’s conserved domains. Validation includes testing for specificity via knockout controls or siRNA knockdown. Commercial VAMP4 antibodies often cite applications in both basic research and diagnostic contexts, aiding in elucidating vesicular transport mechanisms and disease pathology.
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