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产品描述：人蛋白激酶A锚定蛋白(AKAP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AKAP4含量。AKAP4是一种与精子鞭毛功能相关的蛋白，其缺失或减弱会导致精子活力降低，进而引发不育。研究显示，AKAP4在精子鞭毛的纤维鞘和运动调控中起关键作用，其表达异常与多种男性不育症相关。试剂盒检测范围为25 pg/mL-1600 pg/mL，该产品适用于生殖系统发育、生殖细胞功能调控及相关疾病机制的基础研究，例如利用动物模型探索不育症或睾丸发育异常的分子机制，为科研人员提供稳定、特异的AKAP4定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A kinase (PRKA) anchor protein 4 ELISA Kit; A kinase anchor protein 4 ELISA Kit; A kinase anchor protein 82 kDa ELISA Kit; A-kinase anchor protein 4 ELISA Kit; A-kinase anchor protein 82 kDa ELISA Kit; AKAP 4 ELISA Kit; AKAP 82 ELISA Kit; AKAP-4 ELISA Kit; AKAP4 ELISA Kit; AKAP4_HUMAN ELISA Kit; AKAP82 ELISA Kit; FSC 1 ELISA Kit; FSC1 ELISA Kit; hAKAP 82 ELISA Kit; hAKAP82 ELISA Kit; HI ELISA Kit; Major sperm fibrous sheath protein ELISA Kit; p82 ELISA Kit; PRKA 4 ELISA Kit; PRKA4 ELISA Kit; Protein kinase A anchoring protein 4 ELISA Kit; Protein kinase A-anchoring protein 4 ELISA Kit; Testis specific gene HI ELISA Kit
缩写：AKAP4
Uniprot No.：Q5JQC9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 100-114 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-89 | |||||
回收率：
| The recovery of human AKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 92 | 88-87 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.3392.2572.2982.1598001.8131.6761.7451.6064001.1561.1241.1401.0012000.6990.7430.7210.5821000.3870.4150.4010.262500.2970.3040.3010.162250.2150.2090.2120.07300.1370.1410.139 | ||||||
货期：3-5 working days
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