Mouse Angiopoietin 1,ANG-1 ELISA Kit

大包装询价

货号: IPD-E07302m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血管生成素1(ANG-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPT1含量。ANGPT1即血管生成素1，是血管生成素家族成员。其背景在于它在血管生成、维持血管稳定等过程有重要作用。研究机制方面，它主要通过与受体Tie-2结合，激活下游信号通路，调控内皮细胞的存活、迁移及血管成熟等。试剂盒检测范围为1.25 ng/mL-80 ng/mL，该产品可广泛应用于血管生成机制研究、心血管疾病模型评估、肿瘤血管新生相关实验以及发育生物学等领域，为研究ANG-1在组织修复、炎症反应或病理状态下的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Angpt1 ELISA kit; AgptAngiopoietin-1 ELISA kit; ANG-1 ELISA kit
缩写：
ANGPT1
Uniprot No.：
O08538
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
1.25 ng/mL-80 ng/mL
灵敏度：
0.31 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-96
1:2	Average %	95
	Range %	91-99
1:4	Average %	94
	Range %	90-98
1:8	Average %	96
	Range %	89-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
80	2.655	2.756	2.706	2.553
40	2.087	2.188	2.138	1.985
20	1.414	1.435	1.425	1.272
10	0.840	0.851	0.846	0.693
5	0.515	0.506	0.511	0.358
2.5	0.348	0.337	0.343	0.190
1.25	0.266	0.260	0.263	0.110
0	0.153	0.152	0.153

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.