| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CG1I; ZNFN4A1 |
| WB Predicted band size | 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是几篇可能涉及ZNHIT1抗体的参考文献示例(注：部分文献信息为模拟，建议通过学术数据库核实)：
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1. **"ZNHIT1 regulates chromatin remodeling in DNA damage response"**
*Authors: Chen L, Wang X, et al.*
**摘要**：本研究通过免疫沉淀(IP)和Western blot技术，利用ZNHIT1特异性抗体证实了其在DNA损伤修复中与BRCA1复合物的相互作用，揭示了ZNHIT1通过调控染色质重塑影响同源重组修复的分子机制。
2. **"ZNHIT1 deficiency promotes tumorigenesis in hepatocellular carcinoma"**
*Authors: Tanaka K, Suzuki R, et al.*
**摘要**：文章通过免疫组化(IHC)分析肝癌组织中ZNHIT1蛋白表达，发现其表达水平与患者预后相关。使用shRNA敲低ZNHIT1后，Western blot验证了抗体特异性，并证明其缺失通过激活Wnt/β-catenin通路促进肿瘤进展。
3. **"Structural insights into the ZNHIT1-H2A.Z interaction by cryo-EM"**
*Authors: Müller M, Schmidt A, et al.*
**摘要**：利用ZNHIT1抗体进行蛋白纯化，结合冷冻电镜解析了ZNHIT1与组蛋白变体H2A.Z的复合物结构，阐明了其介导染色质动态调控的结构基础。
4. **"ZNHIT1 as a novel biomarker for ovarian cancer chemoresistance"**
*Authors: Li Y, Zhang H, et al.*
**摘要**：通过流式细胞术和免疫荧光检测卵巢癌细胞中ZNHIT1的亚定位，发现其核质移位与顺铂耐药相关，抗体阻断实验进一步验证了其在化疗敏感性中的功能。
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**注意**：上述文献为示例性质，实际引用时请通过PubMed、Google Scholar等平台以“ZNHIT1 antibody”或“ZNHIT1 function”为关键词检索最新研究，并核对作者及期刊信息。
The ZNHIT1 antibody is a research tool designed to detect Zinc Finger HIT-Type Containing 1 (ZNHIT1), a protein encoded by the ZNHIT1 gene. ZNHIT1. also known as p18 hamlet or HSPC324. is a conserved nuclear protein belonging to the HIT (histone triad) superfamily, characterized by a zinc-binding HIT domain. It plays roles in chromatin remodeling, transcriptional regulation, and DNA repair by interacting with histone chaperones and chromatin-modifying complexes. Specifically, ZNHIT1 is a critical component of the SHREC (Snf2 histone linker ejection chaperone) complex, which regulates histone H1 dynamics and heterochromatin formation. It also interacts with the p53 tumor suppressor pathway, influencing cell cycle control and apoptosis.
ZNHIT1 antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study protein expression, localization, and molecular interactions. Dysregulation of ZNHIT1 has been linked to cancers, neurodevelopmental disorders, and premature aging syndromes, making its antibody valuable for investigating disease mechanisms. Researchers validate these antibodies for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial ZNHIT1 antibodies are typically raised in rabbits or mice against synthetic peptides corresponding to conserved regions of the human protein. Proper controls are essential to ensure accurate interpretation in experimental models.
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