| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZFYVE31 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human RUFY4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RUFY4抗体的几篇假设性参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*RUFY4 regulates autophagosome-lysosome fusion in neuronal cells*
**作者**：Smith A, et al.
**摘要**：研究利用RUFY4抗体揭示其在神经元自噬中的作用，发现RUFY4通过结合LC3蛋白促进自噬体与溶酶体融合，缺失会导致神经退行性病变模型中异常蛋白积累。
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2. **文献名称**：*RUFY4 expression correlates with colorectal cancer metastasis*
**作者**：Li Y, et al.
**摘要**：通过免疫组化(RUFY4抗体)分析结直肠癌组织，发现RUFY4高表达与患者转移风险升高相关，其可能通过调控Wnt/β-catenin通路促进肿瘤侵袭。
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3. **文献名称**：*RUFY4 interacts with Rab7 to modulate endosomal trafficking*
**作者**：Chen H, et al.
**摘要**：研究利用RUFY4抗体进行免疫共沉淀实验，证实RUFY4与Rab7蛋白互作，影响内体运输过程，并参与细胞迁移和免疫突触形成。
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4. **文献名称**：*RUFY4 deficiency impairs mitochondrial clearance in macrophages*
**作者**：Wang X, et al.
**摘要**：在巨噬细胞中敲除RUFY4基因(通过Western blot验证抗体特异性)，发现其缺失导致线粒体自噬障碍，加剧炎症反应和败血症模型中的组织损伤。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“RUFY4 antibody”或“RUFY4 function”)。建议结合具体研究方向筛选近期高相关论文。
The RUFY4 antibody is a research tool designed to detect and study the RUN and FYVE domain-containing protein 4 (RUFY4), a member of the RUFY family involved in intracellular trafficking, autophagy, and immune regulation. RUFY4. also known as Rabip4. is characterized by its RUN domain (associated with GTPase interaction) and FYVE domain (zinc finger motif for membrane binding). It plays roles in endosomal sorting, lysosomal function, and signal transduction pathways, with implications in cancer, neurodegenerative diseases, and immune responses.
Antibodies targeting RUFY4 enable researchers to investigate its expression, localization, and interactions in cells or tissues. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies utilizing RUFY4 antibodies have revealed its involvement in autophagosome-lysosome fusion, cytokine secretion, and receptor recycling. Dysregulation of RUFY4 has been linked to pathologies such as metastasis, inflammation, and impaired autophagy in neurodegenerative conditions.
Due to its specificity, the antibody aids in validating RUFY4’s role in disease models and drug discovery. However, its utility depends on proper validation for cross-reactivity and batch consistency. Ongoing research aims to clarify RUFY4’s mechanistic contributions to cellular homeostasis and its potential as a therapeutic target.
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