| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OLA1 |
| Uniprot No | Q9NTK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMPPKKG GDGIKPPPII GRFGTSLKIG IVGLPNVGKS TFFNVLTNSQ ASAENFPFCT IDPNESRVPV PDERFDFLCQ YHKPASKIPA FLNVVDIAGL VKGAHNGQGL GNAFLSHISA CDGIFHLTRA FEDDDITHVE GSVDPIRDIE IIHEELQLKD EEMIGPIIDK LEKVAVRGGD KKLKPEYDIM CKVKSWVIDQ KKPVRFYHDW NDKEIEVLNK HLFLTSKPMV YLVNLSEKDY IRKKNKWLIK IKEWVDKYDP GALVIPFSGA LELKLQELSA EERQKYLEAN MTQSALPKII KAGFAALQLE YFFTAGPDEV RAWTIRKGTK APQAAGKIHT DFEKGFIMAE VMKYEDFKEE GSENAVKAAG KYRQQGRNYI VEDGDIIFFK FNTPQQPKKK |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OLA1重组蛋白的3篇参考文献示例(注：以下文献信息为模拟示例，实际引用时请核实真实来源)：
1. **文献名称**: "OLA1 regulates protein synthesis and stress response through GTPase activity"
**作者**: Zhang, Y. et al.
**摘要**: 研究揭示了OLA1作为GTP酶在细胞应激条件下通过调控翻译起始因子eIF2的磷酸化，抑制全局蛋白质合成的分子机制，并证实重组OLA1蛋白的酶活性在体外实验中显著影响应激颗粒形成。
2. **文献名称**: "Structural insights into human OLA1 ATPase function"
**作者**: Chen, L. et al.
**摘要**: 通过X射线晶体学解析了重组人源OLA1蛋白的三维结构，发现其ATP结合域的关键氨基酸残基对水解活性至关重要，为OLA1在肿瘤细胞代谢异常中的作用提供结构基础。
3. **文献名称**: "Recombinant OLA1 suppresses tumor growth by modulating oxidative stress pathways"
**作者**: Wang, Q. et al.
**摘要**: 利用大肠杆菌表达系统制备重组OLA1蛋白，实验表明其过表达通过激活Nrf2/ARE抗氧化通路抑制乳腺癌细胞增殖，提示OLA1可能作为癌症治疗的潜在靶点。
(注：若需真实文献，建议在PubMed或Web of Science中检索关键词“OLA1 recombinant”或“Obg-like ATPase 1 expression”获取最新研究。)
**Background of OLA1 Recombinant Protein**
OLA1 (Obg-like ATPase 1), also known as Obg homolog ATPase, is a conserved GTPase protein belonging to the Obg family of P-loop NTPases. Initially identified in *E. coli* and yeast, OLA1 plays critical roles in stress response, protein synthesis, and cellular homeostasis. In humans, OLA1 is ubiquitously expressed and localized in the cytoplasm and nucleus. It interacts with multiple signaling pathways, including the mTOR and AMPK pathways, linking energy metabolism to stress adaptation.
Structurally, OLA1 contains a conserved GTPase domain (G domain) and a TGS (ThrRS, GTPase, and Spore) domain, enabling ATP/GTP binding and hydrolysis. Studies suggest OLA1 regulates translation under stress by modulating eIF2α phosphorylation, thereby influencing global protein synthesis. It also participates in the unfolded protein response (UPR) and oxidative stress pathways, acting as a molecular switch to balance cell survival and apoptosis.
OLA1's role in diseases, particularly cancer, is under investigation. Overexpression of OLA1 correlates with poor prognosis in certain cancers, potentially by promoting cell proliferation or chemoresistance. Conversely, reduced OLA1 activity may protect against neurodegenerative disorders linked to protein misfolding.
Recombinant OLA1 protein, produced via bacterial or eukaryotic expression systems, enables functional studies. Purified OLA1 is used to analyze enzymatic activity, substrate interactions, and structural dynamics. Its applications span *in vitro* assays (e.g., ATPase activity), drug screening for cancer or neurodegeneration, and antibody development.
Research on OLA1 recombinant protein continues to uncover its regulatory mechanisms and therapeutic potential, positioning it as a target for modulating stress-related diseases.
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