| 纯度 | >95%SDS-PAGE. |
| 种属 | Escherichia coli |
| 靶点 | sodA |
| Uniprot No | P00448 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-206aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSYTLPSLPYAYDALEPHFDKQTMEIHHTK HHQTYVNNANAALESLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHA NHSLFWKGLKKGTTLQGDLKAAIERDFGSVDNFKAEFEKAAASRFGSGWA WLVLKGDKLAVVSTANQDSPLMGEAISGASGFPIMGLDVWEHAYYLKFQN RRPDYIKEFWNVVNWDEAAARFAAKK |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于sodA重组蛋白研究的参考文献(虚拟示例，仅作格式参考)：
1. **文献名称**: *Heterologous Expression and Characterization of Recombinant Mn-SOD from Bacillus subtilis*
**作者**: Zhang L, et al.
**摘要**: 本研究在大肠杆菌中成功表达了来源于枯草芽孢杆菌的sodA基因，通过镍柱亲和层析纯化获得高纯度Mn-SOD重组蛋白，并验证其抗氧化酶活性及热稳定性。
2. **文献名称**: *Crystal Structure Analysis of sodA-Encoded Manganese Superoxide Dismutase in Staphylococcus aureus*
**作者**: Smith J, et al.
**摘要**: 解析了金黄色葡萄球菌sodA编码的Mn-SOD晶体结构(2.1Å分辨率)，揭示其金属结合位点构象及催化机制，为抗氧化药物设计提供结构基础。
3. **文献名称**: *Functional Rescue of sodA-Deficient E. coli by Recombinant Mn-SOD from Human Pathogens*
**作者**: Kim H, et al.
**摘要**: 通过表达来自结核分枝杆菌和沙门氏菌的sodA重组蛋白，恢复sodA缺陷型大肠杆菌的氧化应激耐受性，证实跨物种Mn-SOD的功能保守性。
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注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science以"sodA recombinant protein"或"Mn-SOD expression"为关键词检索真实文献。
**Background of sodA Recombinant Protein**
The *sodA* gene encodes manganese superoxide dismutase (Mn-SOD), a critical antioxidant enzyme that catalyzes the dismutation of superoxide radicals (O₂⁻) into oxygen and hydrogen peroxide, protecting cells from oxidative stress. Found in bacteria, plants, and mammals, Mn-SOD is localized in mitochondria or bacterial cytoplasm, where it neutralizes reactive oxygen species (ROS) generated during aerobic metabolism. In prokaryotes, *sodA* is essential for survival under oxidative stress, making it a key virulence factor in pathogenic bacteria like *Staphylococcus aureus* and *Mycobacterium tuberculosis*.
Recombinant sodA protein is produced via genetic engineering, typically by cloning the *sodA* gene into expression vectors (e.g., *E. coli*), followed by induction, purification (via affinity chromatography or His-tag systems), and characterization. This approach ensures high yield and purity, enabling functional and structural studies.
Research on sodA recombinant protein focuses on its role in oxidative stress defense, microbial pathogenesis, and potential therapeutic applications. For instance, inhibiting bacterial Mn-SOD has been explored to enhance pathogen susceptibility to host immune responses. In biomedicine, Mn-SOD is studied for its anti-inflammatory and antioxidant properties, with relevance to aging, neurodegenerative diseases, and cancer. Industrial applications include its use in antioxidant formulations or as a stabilizer in biotechnology processes.
Overall, sodA recombinant protein serves as a vital tool for understanding redox biology, microbial resilience, and developing strategies to combat oxidative damage in health and disease.
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