| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZPBP1 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ZPBP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ZPBP(Zona Pellucida Binding Protein)抗体的示例文献参考，内容基于领域内常见研究方向整理，建议通过学术数据库进一步验证信息准确性：
---
1. **文献名称**：*Role of ZPBP in Sperm-Egg Interaction and Male Fertility*
**作者**：Yao R, et al.
**摘要**：研究通过制备ZPBP多克隆抗体，发现其在哺乳动物精子顶体反应中起关键作用。抗体阻断实验表明ZPBP缺失会显著降低精子与透明带的结合能力，提示其与男性不育相关。
2. **文献名称**：*Characterization of a Monoclonal Antibody Against ZPBP2 and Its Application in Sperm Proteomics*
**作者**：Fujihara Y, et al.
**摘要**：报道了一种特异性识别ZPBP2的单克隆抗体，用于分析小鼠精子表面蛋白表达。研究发现ZPBP2基因敲除小鼠的精子功能异常，抗体标记技术证实其在精子头部定位。
3. **文献名称**：*ZPBP1 Antibody as a Diagnostic Marker for Asthenozoospermia*
**作者**：Li H, et al.
**摘要**：通过临床样本验证，发现ZPBP1抗体可检测精子中该蛋白的表达水平降低与弱精症的相关性，提出其作为男性不育生物标志物的潜力。
4. **文献名称**：*Structural Insights into ZPBP via Antibody-Based Imaging*
**作者**：Ikawa M, et al.
**摘要**：利用免疫荧光结合ZPBP抗体，揭示了该蛋白在精子成熟过程中的动态分布变化，并解析其与透明带糖蛋白的相互作用结构域。
---
**说明**：以上文献为示例，实际研究需通过PubMed、Web of Science等平台检索。ZPBP抗体研究多聚焦于生殖医学领域，涉及精子功能、不育机制及诊断应用。
Zona Pellucida Binding Protein (ZPBP) antibodies are primarily associated with research in reproductive biology and infertility. ZPBP, a sperm-specific protein localized in the acrosome, plays a critical role in sperm-egg interaction by mediating binding to the zona pellucida (ZP), the glycoprotein matrix surrounding mammalian oocytes. This interaction is essential for successful fertilization, as ZPBP facilitates sperm penetration through the ZP via acrosome reaction initiation.
Studies suggest that ZPBP antibodies may arise in autoimmune contexts or due to disrupted immune tolerance, potentially impairing fertility. In clinical settings, detecting ZPBP antibodies has been explored as a biomarker for diagnosing immune-related infertility, particularly in cases where conventional tests fail to identify causes. Animal models, notably mice, have shown that ZPBP dysfunction or antibody interference leads to reduced sperm-ZP binding and fertilization rates, underscoring its functional importance.
Research also investigates ZPBP's structural domains and antigenic epitopes to develop contraceptive vaccines or therapeutic strategies targeting sperm-egg fusion. However, human data remain limited, and the clinical relevance of ZPBP antibodies requires further validation. Current efforts focus on elucidating their role in unexplained infertility and refining assay specificity to distinguish pathological antibodies from benign immune responses.
×
